Wobus A M, Grosse R, Schöneich J
Zentralinstitut für Genetik und Kulturpflanzenforschung der AdW der DDR, Gatersleben.
Biomed Biochim Acta. 1988;47(12):965-73.
The effect of nerve growth factor (NGF) on the differentiation pattern of pluripotent undifferentiated mouse embryonic stem cells (ESC) was studied. Morphological analysis of differentiating stem cells and histochemical silver-staining analysis of embryonic carcinoma (P19S1801A1) and embryonic stem cells (ESC-BLC 6) permitted the demonstration of nerve cell differentiation. NGF treatment resulted in an accelerated appearance of neuron-like cells in differentiating embryoid bodies of ESC line BLC 6. By use of the silver-staining test NGF dependent induction of nerve cell lineage was demonstrated. The presented embryonic stem cell model allows to study the time course of differentiation and induction of a special cell lineage of undifferentiated embryonic stem cells by growth factors.
研究了神经生长因子（NGF）对多能未分化小鼠胚胎干细胞（ESC）分化模式的影响。对分化干细胞进行形态学分析，以及对胚胎癌（P19S1801A1）和胚胎干细胞（ESC-BLC 6）进行组织化学银染分析，证实了神经细胞的分化。NGF处理导致ESC系BLC 6分化的胚状体中神经元样细胞加速出现。通过银染试验证明了NGF依赖性神经细胞谱系的诱导。所提出的胚胎干细胞模型有助于研究未分化胚胎干细胞特定细胞谱系的分化时间进程和生长因子诱导情况。
