Iqbal J, Asghar H, Shah S K H, Naeem M, Abbasi S A, Ali R
Appl Opt. 2020 Jun 1;59(16):4927-4932. doi: 10.1364/AO.385932.
In this work, laser-induced breakdown spectroscopy (LIBS) has been used for the quantitative and qualitative analysis of the sage sample using the calibration-free LIBS (CF-LIBS) technique. The sage plasma is generated by focusing the second harmonics (532 nm) of a -switched Nd:YAG laser with a repetition rate of 10 Hz and pulse duration of 5 ns. The emission spectra are recorded using a LIBS 2000 detection system spectrometer consisting of five high-resolution spectrometers covering a wavelength range from 200 to 720 nm. The optical emission spectra of the sage sample reveal the spectral lines of Fe, Ca, Ti, Co, Mn, Ni, and Cr. The plasma temperature and electron number density of the neutral spectral lines of the pertinent elements have been deduced using the Boltzmann plot and Stark-broadening line profile method, with average values 8855±885 and 3.89×10, respectively. The average values of the plasma parameters were used for the quantification of the detected elements in the sample. Based on the calibration-free method, the measured results demonstrate that Fe is the major constituent in the sample, having a percentage concentration of 48.1%, while the remaining elements are Ca, Ti, Co, Mn, Ni, and Cr, with percentage concentrations 0.7%, 5.3%, 8%, 11%, 12.3%, and 14.6%, respectively. This study demonstrates the feasibility of LIBS for the compositional analysis of major and trace elements present in the plant samples and its further applications in medicine.
在这项工作中，激光诱导击穿光谱法（LIBS）已被用于使用无校准LIBS（CF-LIBS）技术对鼠尾草样品进行定量和定性分析。通过聚焦调Q Nd:YAG激光器的二次谐波（532 nm）来产生鼠尾草等离子体，其重复频率为10 Hz，脉冲持续时间为5 ns。使用由五个高分辨率光谱仪组成的LIBS 2000检测系统光谱仪记录发射光谱，该光谱仪覆盖的波长范围为200至720 nm。鼠尾草样品的光学发射光谱揭示了铁、钙、钛、钴、锰、镍和铬的光谱线。利用玻尔兹曼图和斯塔克展宽线轮廓法推导出相关元素中性光谱线的等离子体温度和电子数密度，其平均值分别为8855±885和3.89×10。等离子体参数的平均值用于定量样品中检测到的元素。基于无校准方法，测量结果表明铁是样品中的主要成分，其百分浓度为48.1%，而其余元素为钙、钛、钴、锰、镍和铬，其百分浓度分别为0.7%、5.3%、8%、11%、12.3%和14.6%。本研究证明了LIBS用于植物样品中主要和微量元素成分分析的可行性及其在医学中的进一步应用。
