Khan Abdul Samad, Farooq Imran, Ali Saqib, Khan Soban Qadir, Hakeem Abbas Saeed
Department of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Saudi Pharm J. 2020 Jun;28(6):657-661. doi: 10.1016/j.jsps.2020.04.005. Epub 2020 Apr 28.
To compare effectiveness of ethylenediaminetetraacetic acid (EDTA) and citric acid in removing collagen fiber network covering dentinal tubules of human teeth.
Eighteen dentin discs were divided in three groups; Gp 1: discs received no treatment (control), Gp 2: discs etched with 17% EDTA (pH = 7.1), and Gp 3: discs etched with 6 wt% citric acid (pH = 4.0). Scanning electron microscopy (SEM) was performed to assess collagen fiber removal and X-ray diffraction (XRD) was implemented to analyse crystal peaks of discs.
The SEM analysis demonstrated more collagen removal with EDTA treatment compared to citric acid treated specimens. Grade 6 (81% to 100% fiber removal) was mostly achieved for Gp 2 samples whereas grade 2 (1% to 20% fiber removal) was mostly achieved for Gp 3 samples and inter-group comparisons between these groups were statistically significant (p < 0.05). X-ray diffractogram of control and experimental samples demonstrated absence of calcite phase in experimental groups. The change in peak shapes and intensities were observed and citric acid treated samples revealed more intense peaks than EDTA group.
Our study found 17% EDTA to be more effective in removing collagen fibers when matched with 6% citric acid.
比较乙二胺四乙酸（EDTA）和柠檬酸去除覆盖人牙本质小管的胶原纤维网络的效果。
将18个牙本质盘分为三组；第1组：未处理的牙本质盘（对照组），第2组：用17% EDTA（pH = 7.1）蚀刻的牙本质盘，第3组：用6 wt%柠檬酸（pH = 4.0）蚀刻的牙本质盘。进行扫描电子显微镜（SEM）评估胶原纤维的去除情况，并采用X射线衍射（XRD）分析牙本质盘的晶体峰。
SEM分析表明，与柠檬酸处理的标本相比，EDTA处理能去除更多的胶原。第2组样本大多达到6级（纤维去除率81%至100%），而第3组样本大多达到2级（纤维去除率1%至20%），两组间的组间比较具有统计学意义（p < 0.05）。对照组和实验组样本的X射线衍射图表明实验组中不存在方解石相。观察到峰形和强度的变化，柠檬酸处理的样本显示出比EDTA组更强的峰。
我们的研究发现，与6%柠檬酸相比，17% EDTA在去除胶原纤维方面更有效。
