College of Plant Science, Jilin University, Changchun 130062, PR China.
J Agric Food Chem. 2020 Jul 15;68(28):7316-7323. doi: 10.1021/acs.jafc.0c00333. Epub 2020 Jul 2.
Insects rely on olfaction to locate their host plants by antennae in complex chemical environments. Odorant receptor (OR) genes are thought to play a crucial role in the process. ORs function together with odorant coreceptors to determine the specificity and sensitivity of olfactory reception. The dark black chafer, Motschulsky (Coleoptera: Scarabaeidae), is a destructive underground pest. To understand the molecular basis of olfactory reception, an olfactory-biased expressed odorant receptor HparOR27 and HparOrco (HparOR40) were identified from antennal transcriptome analysis and prediction of the sequence structure. Tissue expression analysis showed that HparOR27 was mainly expressed in adult antennae throughout developmental stages. The functions of HparOR27 were analyzed using the oocyte expression system. HparOR27 was broadly responsive to three host plant volatiles, including hexanal, lauric acid, and tetradecane. Electroantennogram tests confirmed that three ligands were electrophysiologically active in antennae of female adults. A Y-tube olfactometer test indicated that hexanal was a repellent for adults of both sexes. Taken together, our data support the identification of odorant receptors and provide a molecular basis for eco-friendly pest control.
昆虫依靠触角在复杂的化学环境中嗅觉定位其宿主植物。气味受体（OR）基因被认为在这个过程中起着关键作用。OR 与气味共受体一起作用，决定嗅觉感受的特异性和敏感性。暗黑鳃金龟（Motschulsky）（鞘翅目：金龟科）是一种破坏性的地下害虫。为了了解嗅觉接受的分子基础，从触角转录组分析和序列结构预测中鉴定了一个嗅觉偏爱的表达气味受体 HparOR27 和 HparOrco（HparOR40）。组织表达分析表明，HparOR27 在整个发育阶段主要在成年触角中表达。使用卵母细胞表达系统分析了 HparOR27 的功能。HparOR27 对三种宿主植物挥发物，包括己醛、月桂酸和十四烷，表现出广泛的反应性。触角电生理测试证实，三种配体在成年雌虫的触角中具有电生理活性。Y 型嗅觉仪测试表明，己醛对两性成虫均具有驱避作用。综上所述，我们的数据支持了气味受体的鉴定，并为环保型害虫防治提供了分子基础。
