Department of Chemistry, Gomal University, Dera Ismail Khan 29050, Pakistan.
Centre for Materials Science, Islamia College University, Peshawar 25120, Pakistan.
J Proteome Res. 2020 Aug 7;19(8):3201-3210. doi: 10.1021/acs.jproteome.0c00174. Epub 2020 Jul 2.
The thermal stability of purified acid phosphatase from the germinating seedlings of (Jangli halon) was investigated by studying the impact of various thermodynamic parameters [, , Δ° (enthalpy change), Δ° (free energy change), and Δ° (entropy change)] of heat treatment in the temperature range of 55-75 °C. The thermal denaturation of acid phosphatase, assessed by loss in activity, was evidently followed by first-order kinetics, which varies with time and yield during the process of denaturation. The half-life of the enzyme was 693 min at 55 °C. The (activation energy of denaturation) was calculated by the Arrhenius plot (30 kcal mol), and the -value was 17.3 °C. The various thermodynamic parameters studied were as follows: Δ°, the change in enthalpy of inactivation, was 121.93 kJ mol at 55 °C; Δ°, the change in free energy of inactivation, was 110.65 kJ mol at 55 °C; and Δ°, the change in entropy of inactivation, was 34.39 J mol k at 55 °C. This suggests that acid phosphatase activity is thermostable to long heat treatment up to 60 °C.
研究了不同热力学参数（、、Δ°（焓变）、Δ°（自由能变）和Δ°（熵变））对 55-75°C 温度范围内热处理的影响，从而研究了发芽幼苗中纯化的酸性磷酸酶的热稳定性。通过活性丧失评估的酸性磷酸酶热变性显然遵循一级动力学，该动力学随变性过程中的时间和产率而变化。该酶在 55°C 时的半衰期为 693 分钟。通过阿仑尼乌斯图（Arrhenius plot）计算（变性活化能）为 30 kcal mol，-值为 17.3°C。研究的各种热力学参数如下：Δ°，失活焓的变化，在 55°C 时为 121.93 kJ mol；Δ°，失活自由能的变化，在 55°C 时为 110.65 kJ mol；以及 Δ°，失活熵的变化，在 55°C 时为 34.39 J mol K。这表明酸性磷酸酶活性在长达 60°C 的长时间热处理下是热稳定的。
