Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.
Department of Operative Dentistry and Endodontics, Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
Biofouling. 2020 May;36(5):537-548. doi: 10.1080/08927014.2020.1777401. Epub 2020 Jun 19.
biofilms are responsible for oral candidiasis. Fluoxetine is a widely used antidepressant, with certain anti- activities. The antifungal activity of fluoxetine combined with various antifungals against biofilms and oral candidiasis was evaluated in this study. The morphological change in the inhibition of fluoxetine on biofilms was observed using SEM. The interactions between fluoxetine and antifungals against biofilms were evaluated using microdilution checkerboard methods, FICI and the ΔE model. The synergistic combination was tested on the mice model of oral candidiasis. SEM imaging showed fluoxetine inhibited hyphal growth and biofilm formation. Fluoxetine combined with caspofungin exhibited synergistic effects against biofilms. Antagonistic effects occurred when fluoxetine was combined with amphotericin B or terbinafine. Further, the fluoxetine combined with caspofungin significantly reduced the lesion score and CFU of on the murine tongue ( < 0.05), and relieved oral candidiasis of the infected mice.
生物膜是导致口腔念珠菌病的原因。氟西汀是一种广泛应用的抗抑郁药，具有一定的抗-作用。本研究评估了氟西汀与各种抗真菌药物联合应用对生物膜和口腔念珠菌病的抗真菌活性。使用扫描电子显微镜观察氟西汀对生物膜抑制的形态变化。采用微量稀释棋盘法、FICI 和 ΔE 模型评价氟西汀与抗真菌药物对生物膜的相互作用。在口腔念珠菌病小鼠模型上测试协同组合。扫描电子显微镜成像显示氟西汀抑制菌丝生长和生物膜形成。氟西汀与卡泊芬净联合具有协同作用抗生物膜。当氟西汀与两性霉素 B 或特比萘芬联合使用时，会产生拮抗作用。此外，氟西汀与卡泊芬净联合显著降低了小鼠舌部的病变评分和 CFU（<0.05），并缓解了感染小鼠的口腔念珠菌病。
