Environmental Microbiology Unit, Institute for Ecology of Industrial Areas, Katowice, Poland.
Microb Drug Resist. 2021 Feb;27(2):179-189. doi: 10.1089/mdr.2019.0287. Epub 2020 Jun 18.
The study provides data on antibiotic resistance as well as the virulence characteristics of isolated from raw and treated wastewater. The isolates were identified as by 16S rRNA gene sequencing. In the analyzed strains, high frequency for the following genes was observed: , B, and D. The presence of A and B genes was not found in any strain. The higher frequency of the investigated genes was observed in strains from raw wastewater (RW). The strains of showed multiple antibiotic resistance evaluated by the disk diffusion method. Multiple antibiotic resistance indices ranged from 0.36 to 0.69. Susceptibility to six heavy metals (Cd, Zn, Cu, Co, Mn, and Ni) was recorded for all the isolates. The order of metal resistance of was Co > Cu > Zn > Cd > Ni > Mn. All the strains of showed β-hemolytic activity. Enzymes of amylase, cellulase, and lipase were produced by all isolates. Only the strains from RW had the ability to form biofilms and showed motility. The obtained results indicate that wastewater is a potential source and/or reservoir of virulent and multidrug-resistant as "high-risk isolates."
本研究提供了从原水和处理废水中分离的 对抗生素耐药性和毒力特征的数据。通过 16S rRNA 基因测序鉴定分离株为 。在所分析的菌株中，观察到以下基因的高频： 、B 和 D。在任何菌株中均未发现 A 和 B 基因。在原水（RW）来源的菌株中观察到研究基因的更高频率。通过纸片扩散法评估，显示出对多种抗生素的耐药性。多重抗生素耐药指数范围为 0.36 至 0.69。所有分离株均对六种重金属（Cd、Zn、Cu、Co、Mn 和 Ni）表现出敏感性。 的金属耐药顺序为 Co > Cu > Zn > Cd > Ni > Mn。所有 的菌株均表现出β-溶血活性。所有分离株均产生淀粉酶、纤维素酶和脂肪酶。只有 RW 来源的菌株具有形成生物膜和运动的能力。研究结果表明，废水是具有毒力和多药耐药性的 的潜在来源和/或储库，被称为“高风险分离株”。
