College of Life Science of Longyan University, Longyan, 364012, China.
Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, China.
Virol J. 2020 Jun 18;17(1):79. doi: 10.1186/s12985-020-01345-7.
A prevalent PEDV strain, designated FJLY06, was isolated from Fujian, China. The four of structural genes sequences of PEDV obtained were analyzed to determine their phylogenetic relationships and homology respectively, revealing that FJLY06 was highly homologous to virulent PEDV strains. The four structural genes all differed genetically from the vaccine strain CV777. The sequence alignment results further showed that N, M and E genes of Chinese PEDV strains is highly conserved. Compared with the vaccine strain CV777, 8 mutations were detected in COE of FJLY06 S gene. The recombination analysis revealed FJLY06 is similar to other pandemic strains in China with a variant S gene, and maybe a reason for recent vaccination failures.
从中国福建分离到一种流行的猪流行性腹泻病毒（PEDV）毒株，命名为 FJLY06。对获得的 PEDV 的 4 个结构基因序列进行分析，以确定它们的系统进化关系和同源性，结果表明 FJLY06 与强毒 PEDV 株高度同源。这 4 个结构基因在遗传上均与疫苗株 CV777 不同。序列比对结果进一步表明，中国 PEDV 株的 N、M 和 E 基因高度保守。与疫苗株 CV777 相比，FJLY06 的 S 基因 COE 区检测到 8 个突变。重组分析显示，FJLY06 与中国其他流行株相似，具有变异的 S 基因，这可能是最近疫苗接种失败的一个原因。
