盐对来自T4噬菌体感染的大肠杆菌的RNA聚合酶转录T7 DNA的影响。
Effect of salt on the transcription of T7 DNA by RNA polymerase from T4 phage-infected E.coli.
作者信息
Stevens A
出版信息
Nucleic Acids Res. 1977 Apr;4(4):877-82. doi: 10.1093/nar/4.4.877.
Abstract
Transcription of T7 DNA by T4 core enzyme with host sigma is more sensitive to KCI than that by host core enzyme with host sigma. When salt is added after initiation of RNA chains has occurred, it is not inhibitory. Salt affects the binding of T4 enzyme to T7 DNA to the same degree as the binding of host enzyme. Active preinitiation complex formation is inhibited more by salt with the T4 enzyme and the inhibition is temperature-dependent.
摘要
T4核心酶与宿主σ因子对T7 DNA的转录比宿主核心酶与宿主σ因子对T7 DNA的转录对KCl更为敏感。当在RNA链起始后添加盐时，它没有抑制作用。盐对T4酶与T7 DNA结合的影响程度与宿主酶的结合相同。活性起始前复合物的形成在T4酶存在时受盐的抑制作用更强，且这种抑制作用是温度依赖性的。
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