Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
Zhejiang Pukang Biotechnology Co., LTD., Hangzhou, China.
Virus Res. 2020 Sep;286:198067. doi: 10.1016/j.virusres.2020.198067. Epub 2020 Jun 15.
The morbidity and mortality of coxsackievirus A10 (CVA10)-associated hand, foot, and mouth disease (HFMD) have been increasing in recent years, while few studies on the vaccine and animal model of CVA10 have been reported. Here, we first established a CVA10-infected gerbil model and employed it to evaluate the immunoprotective effect of an inactivated CVA10 vaccine. The results showed that gerbils up to the age of 14 days were fully susceptible to CVA10, and all died within five days post-infection by intraperitoneal inoculation. Lethargy, wasting, hind-limb paralysis, and even death could be observed in the CVA10-infected gerbils. Pathological examination suggested that CVA10 has a strong tropism toward muscle tissue, and muscle bundle fracture and muscular fibers necrosis were observed in the limb muscles. Additionally, active immunization results showed that gerbils immunized with the inactivated CVA10 vaccine were 100 % protected from lethal CVA10 challenge. The antisera from vaccinated gerbils also showed high neutralizing titers against CVA10. Based on these results, the CVA10-infected gerbil model was a suitable tool for analyzing the pathogenesis of CVA10 and assessing the protective efficacy of CVA10 candidate vaccines.
近年来，柯萨奇病毒 A10（CVA10）相关手足口病（HFMD）的发病率和死亡率一直在上升，而关于 CVA10 的疫苗和动物模型的研究却很少报道。在这里，我们首次建立了 CVA10 感染沙鼠模型，并将其用于评估 CVA10 灭活疫苗的免疫保护效果。结果表明，14 日龄以内的沙鼠对 CVA10 完全易感，腹腔接种后 5 天内全部死亡。感染 CVA10 的沙鼠表现出嗜睡、消瘦、后肢瘫痪，甚至死亡。病理检查表明 CVA10 对肌肉组织具有很强的嗜性，在四肢肌肉中观察到肌束断裂和肌纤维坏死。此外，主动免疫结果表明，用 CVA10 灭活疫苗免疫的沙鼠可 100%免受致死性 CVA10 攻击。接种沙鼠的血清也显示出针对 CVA10 的高中和滴度。基于这些结果，CVA10 感染沙鼠模型是分析 CVA10 发病机制和评估 CVA10 候选疫苗保护效果的合适工具。
