Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, WB, India.
Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, WB, India
Biol Open. 2020 Jul 21;9(7):bio053322. doi: 10.1242/bio.053322.
Checkpoint activation and gene expression modulation represent key determinants of cellular survival in adverse conditions. The former is regulated by cyclin-dependent kinases (CDKs) while the latter can be controlled by mitogen-activated protein kinases (MAPKs). Association between cell-cycle progression and MAPK-dependent gene expression exists in cells growing in optimal environments. While MAPK-mediated regulation of the cell cycle is well characterised, the reciprocal influence of mitotic CDK on stress response is not well studied. We present evidence that CDK activity can regulate the extent of MAPK activation in cells. We show that increasing or decreasing mitotic CDK (Cdc2) activity in cells can affect the activation of stress responsive MAPK (Spc1) even in the absence of stress stimuli. Our results indicate that the strong correlation between Cdc2 activity and Spc1 MAPK-activity in is important in regulating mitotic timing.This article has an associated First Person interview with the first author of the paper.
检查点激活和基因表达调控是细胞在不利条件下存活的关键决定因素。前者受细胞周期蛋白依赖性激酶（CDKs）调控，而后者可受丝裂原活化蛋白激酶（MAPKs）调控。在最佳环境中生长的细胞中，细胞周期进展与 MAPK 依赖性基因表达之间存在关联。虽然 MAPK 介导的细胞周期调控已得到很好的描述，但有丝分裂 CDK 对应激反应的反向影响尚未得到很好的研究。我们提供的证据表明，CDK 活性可以调节细胞中 MAPK 激活的程度。我们表明，在没有应激刺激的情况下，增加或减少 细胞中的有丝分裂 CDK（Cdc2）活性可以影响应激反应性 MAPK（Spc1）的激活。我们的结果表明，在 中 Cdc2 活性和 Spc1 MAPK 活性之间的强相关性对于调节有丝分裂时间很重要。本文有一篇与该论文第一作者的相关第一人称采访。
