Kovalenko A G, Votselko S K
Prikl Biokhim Mikrobiol. 1977 Jan-Feb;13(1):46-54.
By the methods of biogel R-10, R-30, R-60 and R-200 gel filtration, sedimentation analysis and IR-spectroscopy polysaccharides isolated from yeast Candida tropicalis 3b, C. arborea KAM-1 and a commercial yeast extract (Merck) were investigated. The effect of the preparations on TMV was also studied. Polysaccharides consisted primarily of glucanes with a molecular weight of 10000-16500. The yeast extract preparation also included mannane with a molecular weight of 48000. IV-spectra of the purified preparations were in the main identical and were typical of polysaccharides. The mannane containing preparation inhibited TMV more significantly that glucane containing preparations. The interaction between physical parameters, structure and biological activity of the biopolymers tested is discussed.
采用生物凝胶R - 10、R - 30、R - 60和R - 200凝胶过滤、沉降分析和红外光谱法，对从热带假丝酵母3b、树木假丝酵母KAM - 1和市售酵母提取物（默克公司）中分离得到的多糖进行了研究。还研究了这些制剂对烟草花叶病毒（TMV）的影响。多糖主要由分子量为10000 - 16500的葡聚糖组成。酵母提取物制剂还包括分子量为48000的甘露聚糖。纯化制剂的红外光谱基本相同，是多糖的典型光谱。含甘露聚糖的制剂比含葡聚糖的制剂对TMV的抑制作用更显著。讨论了所测试生物聚合物的物理参数、结构和生物活性之间的相互作用。
