Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
Division of Reproductive Biotechnology and Innovation, Center for Animal Resources and Development (CARD), Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
Exp Anim. 2020 Nov 12;69(4):423-429. doi: 10.1538/expanim.20-0042. Epub 2020 Jun 17.
The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 h. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice.
将两细胞胚胎进行冷藏是一种在不运输活体动物的情况下运输基因工程小鼠的有用技术。然而，随着储存时间的延长，冷藏胚胎的发育能力会下降。因此，运输的胚胎在到达时必须能够立即转移到受体小鼠中。冷冻保存冷藏运输的胚胎可以提高胚胎移植时间安排的灵活性。在本文中，我们检查了在冷藏温度下冷藏 0、24、48、72 或 96 小时后，两细胞期玻璃化冷冻解冻小鼠胚胎的活力和发育能力。冷藏后的玻璃化冷冻解冻胚胎的活力与未经冷藏的玻璃化冷冻解冻胚胎相当。经冷藏后的玻璃化冷冻解冻胚胎也通过胚胎移植正常发育为幼仔。此外，还从旭川医科大学进行的冷藏胚胎运输中获得了活幼仔。总之，冷藏胚胎可用于基因工程小鼠的运输和存档。
