The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, PR China.
Shenzhen Baoan People's Hospital (Group), The Second People's Hospital, Shenzhen, Guangdong 518101, China.
Folia Histochem Cytobiol. 2020;58(2):127-134. doi: 10.5603/FHC.a2020.0011. Epub 2020 Jun 18.
Oxidative stress and cell apoptosis have both been suggested to be closely associated with the pathogenesis of Parkinson's disease (PD). Previously, bisdemethoxycurcumin (BDMC) has been shown to exhibit several desirable characteristics as a candidate neuroprotective agent, including antioxidant and anti-inflammatory activities in the nervous system. However, whether BDMC can exert cell-protective roles in an in vitro model of PD remains unknown.
SH-SY5Y cells were pretreated with BDMC, with or without AG490 and SI-201, for 30 min, followed by a co-incubation with rotenone for 24 h. Subsequently, a cell viability assay and western blotting was performed, and SOD and GSH activities were analyzed.
The results revealed that the pretreatment with BDMC enhanced the cell survival, antioxidative stress capacity and the phosphorylation levels of JAK/STAT3 in SH-SY5Y cells treated with rotenone. However, following the incubation with AG490 and SI-201, inhibitors of the JAK/STAT3 signaling pathway, BDMC was unable to exert cell-protective roles in SH-SY5Y cells treated with rotenone.
In conclusion, the results suggested that BDMC may exert a cell-protective role in SH-SY5Y cells in vitro via JAK2/STAT3 signaling, thus suggesting the possible application of BDMC for the treatment of neurodegenerative diseases related to JAK2/STAT3 signaling.
氧化应激和细胞凋亡都与帕金森病（PD）的发病机制密切相关。此前，双去甲氧基姜黄素（BDMC）已被证明具有多种作为候选神经保护剂的理想特性，包括在神经系统中具有抗氧化和抗炎活性。然而，BDMC 是否能在 PD 的体外模型中发挥细胞保护作用尚不清楚。
SH-SY5Y 细胞用 BDMC 预处理 30min，然后与 AG490 和 SI-201 共同孵育 24h。然后进行细胞活力测定和 Western blot 分析，以及 SOD 和 GSH 活性分析。
结果表明，BDMC 预处理增强了经鱼藤酮处理的 SH-SY5Y 细胞的细胞存活率、抗氧化应激能力和 JAK/STAT3 的磷酸化水平。然而，在用 AG490 和 SI-201 孵育后，JAK/STAT3 信号通路的抑制剂，BDMC 无法在经鱼藤酮处理的 SH-SY5Y 细胞中发挥细胞保护作用。
总之，这些结果表明，BDMC 可能通过 JAK2/STAT3 信号通路在体外的 SH-SY5Y 细胞中发挥细胞保护作用，这表明 BDMC 可能用于治疗与 JAK2/STAT3 信号通路相关的神经退行性疾病。
