Spiegelhauer Malene R, Kupcinskas Juozas, Johannesen Thor B, Urba Mindaugas, Skieceviciene Jurgita, Jonaitis Laimas, Frandsen Tove H, Kupcinskas Limas, Fuursted Kurt, Andersen Leif P
Department of Clinical Microbiology, Rigshospitalet, Henrik Harpestrengs Vej 4A, 2100 Copenhagen, Denmark.
Department of Gastroenterology, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009 Kaunas, Lithuania.
J Clin Med. 2020 Jun 16;9(6):1882. doi: 10.3390/jcm9061882.
is a common colonizer of the human stomach, and long-term colonization has been related to development of atrophic gastritis, peptic ulcers and gastric cancer. The increased gastric pH caused by colonization, treatment with antibiotics or proton pump inhibitors (PPI) may allow growth of other bacteria. Previous studies have detected non- bacteria in stomach biopsies, but no conclusion has been made of whether these represent a transient contamination or a persistent microbiota. The aim of this study was to evaluate the transient and persistent bacterial communities of gastric biopsies. The washed or unwashed gastric biopsies were investigated by cultivation and microbiota analysis (16S rRNA gene-targeted amplicon sequencing) for the distribution of and other non- bacteria. The number of cultured non- bacteria decreased in the washed biopsies, suggesting that they might be a transient contamination. No significant differences in the bacterial diversity were observed in the microbiome analysis between unwashed and washed biopsies. However, the bacterial diversity in biopsies shown -positive and -negative were significantly different, implying that is the major modulator of the gastric microbiome. Further large-scale studies are required to investigate the transient and persistent gastric microbiota.
是人类胃部常见的定植菌，长期定植与萎缩性胃炎、消化性溃疡和胃癌的发生有关。由定植、抗生素治疗或质子泵抑制剂（PPI）引起的胃内pH值升高可能会使其他细菌生长。先前的研究在胃活检组织中检测到了非幽门螺杆菌，但对于这些细菌是代表短暂污染还是持续存在的微生物群尚无定论。本研究的目的是评估胃活检组织中的短暂和持续细菌群落。通过培养和微生物群分析（靶向16S rRNA基因的扩增子测序）对洗涤或未洗涤的胃活检组织进行研究，以分析幽门螺杆菌和其他非幽门螺杆菌的分布情况。洗涤后的活检组织中培养出的非幽门螺杆菌数量减少，这表明它们可能是短暂污染。在微生物组分析中，未洗涤和洗涤后的活检组织之间未观察到细菌多样性的显著差异。然而，幽门螺杆菌阳性和阴性的活检组织中的细菌多样性存在显著差异，这意味着幽门螺杆菌是胃微生物群的主要调节因子。需要进一步的大规模研究来调查短暂和持续的胃微生物群。
