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一种用于蝇类(双翅目)口器扫描电子显微镜观察的简单制备方法。

A simple preparatory method for scanning electron microscopy of the mouthparts of muscoid flies (diptera).

作者信息

Kovács F S

机构信息

Department of General Zoology and Parasitology, University of Veterinary Sciences, Budapest, Hungary.

出版信息

Acta Biol Hung. 1988;39(4):441-7.

PMID:3256181
Abstract

A Scanning electron microscopic preparation method is described, especially for muscoid flies also useful for the preparation of other chitinous structures of insects. Carbon-tetrachloride was used to maintain the proper positions of the proboscis and the labella rendering a better view of the fine structures. Specimens were cleaned by a bioactive washing powder, then air-dried prior to fixing them on stubs by a reliable home-made adhesive.

摘要

本文描述了一种扫描电子显微镜标本制备方法,该方法特别适用于蝇类,也可用于制备昆虫的其他几丁质结构。使用四氯化碳来固定喙和唇瓣的位置,以便更好地观察精细结构。标本先用生物活性洗衣粉清洗,然后在空气中干燥,再用一种可靠的自制粘合剂固定在短柱上。

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