School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, Tamil Nadu, 613401, India.
Research Centre, Department of Botany and Microbiology, Lady Doak College, Madurai, Tamil Nadu, 625002, India.
Environ Sci Pollut Res Int. 2020 Oct;27(29):36498-36509. doi: 10.1007/s11356-020-09641-z. Epub 2020 Jun 19.
Lipase enzyme has a critical role in deinking process along with other lignocellulosic enzymes. In this paper, we try to demonstrate the role of lipase in the enzyme cocktail used for enzymatic deinking. For this, we identified a potential lipolytic bacterium, Pseudomonas mendocina ED9 isolated from elephant dung with a molecular weight of 35 kDa. During the Box-Benhken model optimization, a maximum lipase activity of 105.12 U/g, which was 12.36-fold higher than the initial enzyme activity and 1.3-fold higher than the activity obtained during the Plackett Burman design, was achieved. A maximum lipase activity of 105.12 U/g was obtained after optimization. Ammonium sulphate (60%) precipitation resulted in a specific activity of 68.19 U/mg with a 1.4-fold purification and yield of 64%. Lipase from P. mendocina ED9 exhibited a Km of 0.5306 mM and Vmax of 25.0237 μmol/min/mg. A Δ brightness of approximately 14.5% were achieved during the enzymatic deinking using cocktail comprised of cellulase, xylanase and lipase. This reports the significant role and efficacy of lipase in enzyme cocktails for deinking applications. This formulation will reduce the pollution and environmental toxicity of conventional chemical deinking.
脂肪酶在脱墨过程中与其他木质纤维素酶一起起着关键作用。在本文中，我们试图证明脂肪酶在用于酶法脱墨的酶混合物中的作用。为此，我们从大象粪便中分离出一种潜在的脂肪分解菌 Pseudomonas mendocina ED9，其分子量为 35 kDa。在 Box-Benhken 模型优化过程中，获得了最大脂肪酶活为 105.12 U/g，比初始酶活高 12.36 倍，比 Plackett Burman 设计获得的酶活高 1.3 倍。优化后获得最大脂肪酶活 105.12 U/g。硫酸铵（60%）沉淀得到比活为 68.19 U/mg，纯化倍数为 1.4 倍，产率为 64%。P. mendocina ED9 的脂肪酶的 Km 值为 0.5306 mM，Vmax 值为 25.0237 μmol/min/mg。使用包含纤维素酶、木聚糖酶和脂肪酶的鸡尾酒进行酶法脱墨可实现约 14.5%的Δ亮度。这表明脂肪酶在酶法脱墨中的作用和效果显著。这种配方将减少传统化学脱墨的污染和环境毒性。
