巴西塞阿拉州美洲利什曼病的流行病学研究。利什曼原虫分离株的分子特征分析。

Epidemiological studies on American leishmaniasis in Ceará State, Brazil. Molecular characterization of the Leishmania isolates.

作者信息

Vasconcelos I A, Vasconcelos A W, Momen H, Grimaldi G, Alencar J E

机构信息

Nycleo de Medicina Tropical do Centro de Ciencias da Saude da Universidade Federal do Ceará, Rua Alexandre Barauna, Fortaleza, Brazil.

出版信息

Ann Trop Med Parasitol. 1988 Dec;82(6):547-54. doi: 10.1080/00034983.1988.11812290.

DOI:10.1080/00034983.1988.11812290

PMID:3256276

Abstract

Two methods of molecular characterization, using monoclonal antibodies and enzyme electrophoresis, were employed in the identification of 36 stocks of Leishmania isolated from human and canine cases of American visceral (AVL) and cutaneous (ACL) leishmaniases in the northern part of Ceará State, Brazil. Molecular homogeneous strains of Leishmania donovani (chagasi) isolated from both human and canine hosts were detected in 14 municipalities. Two more parasite species, L. braziliensis braziliensis and L. mexicana amazonensis, were also detected in the state. The implication of these results with respect to both the clinical and epidemiological data are discussed.

摘要

采用单克隆抗体和酶电泳两种分子鉴定方法，对从巴西塞阿拉州北部人类和犬类美洲内脏利什曼病（AVL）及皮肤利什曼病（ACL）病例中分离出的36株利什曼原虫进行鉴定。在14个市检测到从人类和犬类宿主中分离出的分子均一的杜氏利什曼原虫（恰加斯亚种）菌株。该州还检测到另外两种寄生虫，即巴西利什曼原虫巴西亚种和亚马逊利什曼原虫墨西哥亚种。讨论了这些结果在临床和流行病学数据方面的意义。