Baleeiro Carolina O, Paranhos-Silva Moacir, dos Santos Juliana C, Oliveira Geraldo G S, Nascimento Eliane Goes, de Carvalho Lain Pontes, dos-Santos Washington L C
Centro de Pesquisas Gonçalo Moniz-Fiocruz, Fundação Oswaldo Cruz, Rua Waldemar Falcão 121, 40295-001 Salvador (BA), Brazil.
Vet Parasitol. 2006 Jun 30;139(1-3):21-8. doi: 10.1016/j.vetpar.2006.02.033. Epub 2006 Mar 29.
In this study, humoral (circulating anti-Leishmania antibodies) and cellular (Montenegro's skin test) immune responses of dogs from an endemic area of visceral leishmaniosis were tested using Leishmania chagasi, Leishmania amazonensis and Leishmania braziliensis antigens. The antibody response was tested in three animal groups, selected according to their anti-L. chagasi antibody activity, as measured by ELISA in the serum: 19 negative (O.D. below 0.30), seven with undefined (O.D. between 0.40 and 0.70) and 12 positive (O.D. above 1.0) ELISA result. In the group of animals with positive ELISA, the antibody activity against L. chagasi antigens (mean O.D.=1.31) was significantly higher (ANOVA, P<0.01) than against L. amazonensis (mean O.D.=0.88) or L. braziliensis (mean O.D.=0.87) antigens. The Montenegro's skin test results obtained with L. chagasi and L. braziliensis antigens showed a fair agreement (kappa=0.309). The same was observed when antigens from L. braziliensis and L. amazonensis were compared (kappa=0.374), whereas a moderate agreement between the results from tests performed with L. chagasi and L. amazonensis antigens was observed (kappa=0.530). The induration areas obtained with L. braziliensis antigen were smaller than those obtained with the other antigens. The data presented herein indicate that the use of antigens from different Leishmania species may interfere with the results of the immunological tests performed in dogs in an endemic area of visceral leishmaniosis.
在本研究中，使用恰加斯利什曼原虫、亚马逊利什曼原虫和巴西利什曼原虫抗原，对来自内脏利什曼病流行地区的犬的体液免疫反应（循环抗利什曼原虫抗体）和细胞免疫反应（蒙氏皮肤试验）进行了检测。根据血清中通过酶联免疫吸附测定法（ELISA）测得的抗恰加斯利什曼原虫抗体活性，将三个动物组的抗体反应进行了检测：19只为阴性（光密度低于0.30），7只为不确定（光密度在0.40至0.70之间），12只为阳性（光密度高于1.0），ELISA结果呈阳性。在ELISA呈阳性的动物组中，针对恰加斯利什曼原虫抗原的抗体活性（平均光密度=1.31）显著高于针对亚马逊利什曼原虫（平均光密度=0.88）或巴西利什曼原虫（平均光密度=0.87）抗原的抗体活性（方差分析，P<0.01）。用恰加斯利什曼原虫和巴西利什曼原虫抗原进行蒙氏皮肤试验的结果显示出一定的一致性（kappa=0.309）。比较巴西利什曼原虫和亚马逊利什曼原虫的抗原时也观察到了同样的情况（kappa=0.374），而在用恰加斯利什曼原虫和亚马逊利什曼原虫抗原进行的试验结果之间观察到了中等程度的一致性（kappa=0.530）。用巴西利什曼原虫抗原获得的硬结面积小于用其他抗原获得的硬结面积。本文提供的数据表明，使用来自不同利什曼原虫物种的抗原可能会干扰在内脏利什曼病流行地区对犬进行的免疫检测结果。
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