Tea Science Research Institute, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Tea Science Research Institute, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Genomics. 2020 Sep;112(5):3497-3503. doi: 10.1016/j.ygeno.2020.06.025. Epub 2020 Jun 17.
Nitrogen (N) element is essential nutrient, and affect metabolism of secondary metabolites in higher plants. Ascorbate peroxidase (APX) plays an important role in ascorbic acid (AsA) metabolism of tea plant. However, the roles of cytosolic ascorbate peroxidase 1 (CsAPX1) in AsA metabolism under N deficiency stress in tea plant remains unclear in detail. In this work, nitrogen regulatory protein P-II (CsGLB1) and CsAPX1 were identified by isobaric tags for relative and absolute quantitation (iTRAQ) from tea plant. The cell growth rates in transgenic Escherichia coli overexpressing CsAPX1 and CsGLB1 were higher than empty vector under N sufficiency condition. Phenotype of shoots and roots, AsA accumulation, and expression levels of AtAPX1 and AtGLB1 genes were changed in transgenic Arabidopsis hosting CsAPX1 under N deficiency stress. These findings suggested that cytosolic CsAPX1 acted a regulator in AsA accumulation through cooperating with GLB1 under N deficiency stress in tea plant.
氮（N）元素是必需营养元素，影响高等植物次生代谢物的代谢。抗坏血酸过氧化物酶（APX）在茶树的抗坏血酸（AsA）代谢中发挥重要作用。然而，在茶树氮缺乏胁迫下，细胞质抗坏血酸过氧化物酶 1（CsAPX1）在 AsA 代谢中的作用还不清楚。在这项工作中，通过等重同位素标记相对和绝对定量（iTRAQ）技术，从茶树中鉴定出氮调节蛋白 P-II（CsGLB1）和 CsAPX1。在氮充足条件下，过表达 CsAPX1 和 CsGLB1 的转基因大肠杆菌的细胞生长速率高于空载体。在氮缺乏胁迫下，转基因拟南芥中 CsAPX1 的表达水平发生变化，表现出 shoot 和 root 的表型、AsA 积累以及 AtAPX1 和 AtGLB1 基因的表达水平发生变化。这些发现表明，在茶树氮缺乏胁迫下，细胞质 CsAPX1 通过与 GLB1 合作，在 AsA 积累中起调节作用。
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