Lindahl M, Andersson O, Ripe E, Holm G
Department of Lung Medicine, Huddinge University Hospital, Sweden.
Br J Dis Chest. 1988 Oct;82(4):386-93. doi: 10.1016/0007-0971(88)90093-9.
BAL and blood mononuclear cells and their reactivity to Kveim antigen, tuberculin and concanavalin A (Con A) were studied in nine patients with different clinical stages of sarcoidosis. After separation by plastic adherence, non-adherent cells (mainly lymphocytes) were admixed with 10% autologous adherent cells (monocytes/macrophages). After 3 and 6 days' culture with Kveim antigen (1, 10, 100 micrograms/ml), PPD tuberculin (2.5 micrograms/ml) and Con A (10, 20, 40 micrograms/ml) stimulation was measured as incorporation of 14C-thymidine into DNA. Except for occasional reactions the study did not show any unitary significant increase in lymphocyte response to the different concentrations of Kveim antigen in either BAL or blood. For Con A there was a weaker response by BAL-mononuclear cells with no difference between 3 and 6 days, compared with blood where there was an early peak. The lymphocyte reaction to PPD was weak with no difference between blood and BAL.
对9例处于结节病不同临床阶段的患者的支气管肺泡灌洗（BAL）液及血液中的单核细胞及其对克维姆抗原、结核菌素和刀豆蛋白A（Con A）的反应性进行了研究。通过塑料贴壁分离后，将非贴壁细胞（主要是淋巴细胞）与10%的自体贴壁细胞（单核细胞/巨噬细胞）混合。在用克维姆抗原（1、10、100微克/毫升）、PPD结核菌素（2.5微克/毫升）和Con A（10、20、40微克/毫升）刺激培养3天和6天后，通过测量14C-胸腺嘧啶核苷掺入DNA的量来检测刺激反应。除偶尔有反应外，该研究未显示BAL液或血液中淋巴细胞对不同浓度克维姆抗原的反应有任何一致的显著增加。对于Con A，BAL单核细胞的反应较弱，3天和6天之间无差异，而血液中的反应有一个早期峰值。淋巴细胞对PPD的反应较弱，血液和BAL液之间无差异。
