Eladl Abdelfattah H, Mosad Samah M, El-Shafei Reham A, Saleh Rasha M, Ali Hanaa S, Badawy Basma M, Elshal Mohamed F
Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Comp Immunol Microbiol Infect Dis. 2020 Oct;72:101505. doi: 10.1016/j.cimid.2020.101505. Epub 2020 Jun 7.
This study was conducted to assess the comparative effects of a mixed herbal extract (MHE) containing Ocimum sanctum, Withania somnifera, Emblica officinalis, Tinospora cordifolia, Mangifera indica, and Asphaltum (shilajit) on infectious bursal disease virus (IBDV)-vaccinated (VAC) chickens infected with IBDV and avian influenza virus (AIV) H9N2. The experiment included three groups (G1-G3): G1, the negative control group; G2, the VAC + challenged (Ch) group; and G3, the VAC + Ch + MHE group. MHE was orally administered continuously for 5 weeks post-vaccination (PV) with IBDV at 12 days of age, and the chicks were simultaneously challenged with virulent IBDV (intraocularly) and AIV H9N2 (intranasally) at 21 days PV. Blood and tissue samples as well as tracheal and cloacal swabs were gathered at different times PV and post-challenge. Immunological and haematological parameters, histopathological lesions, relative organ weights and final live weights revealed significant differences (P ≤ 0.05) between G2 and G3 groups. Furthermore, in the G3 group, the protection rates, ELISA and HI titers and CD4+/CD8+ ratio were significantly increased, whereas viral shedding titers and the heterophil/lymphocyte ratio were decreased. In conclusion, the oral administration of the mixed herbal extract for 5 weeks can stimulate the immune response to IBDV vaccination and relieves the pathogenicity of an AIV H9N2 and IBDV co-infection in chickens.
本研究旨在评估一种混合草药提取物（MHE）对感染传染性法氏囊病病毒（IBDV）和H9N2亚型禽流感病毒（AIV）的IBDV疫苗接种（VAC）鸡的比较效果。该混合草药提取物包含圣罗勒、印度人参、余甘子、心叶锡生藤、芒果和沥青（希拉季特）。实验包括三组（G1 - G3）：G1为阴性对照组；G2为VAC + 攻毒（Ch）组；G3为VAC + Ch + MHE组。在12日龄用IBDV疫苗接种后连续5周口服MHE，在接种后21天同时用强毒IBDV（眼内接种）和AIV H9N2（鼻内接种）对雏鸡进行攻毒。在接种后和攻毒后的不同时间收集血液和组织样本以及气管和泄殖腔拭子。免疫和血液学参数、组织病理学病变、相对器官重量和最终活体重显示G2组和G3组之间存在显著差异（P≤0.05）。此外，在G3组中，保护率、ELISA和HI效价以及CD4+/CD8+比值显著增加，而病毒排毒效价和嗜异性粒细胞/淋巴细胞比值降低。总之，连续5周口服混合草药提取物可刺激对IBDV疫苗接种的免疫反应，并减轻AIV H9N2和IBDV共同感染对鸡的致病性。
