School of Criminal Justice, Faculty of Law, Criminal Justice and Public Administration, University of Lausanne, Lausanne, Dorigny, Switzerland; Forensic Genetics Unit, University Center of Legal Medicine Lausanne and Geneva, Lausanne, Switzerland.
School of Criminal Justice, Faculty of Law, Criminal Justice and Public Administration, University of Lausanne, Lausanne, Dorigny, Switzerland.
Forensic Sci Int Genet. 2020 Sep;48:102334. doi: 10.1016/j.fsigen.2020.102334. Epub 2020 Jun 11.
This paper presents a methodology allowing identification of the variables associated with transfer, persistence or recovery of DNA traces that have the most significant impact on the result of an evaluation measured through a likelihood ratio (LR). It builds on a case scenario involving trace DNA recovered from knife handles where the prosecution alleges that the person of interest (POI) stabbed a victim whereas the defence claims that the POI has nothing to do with the incident and the victim was stabbed by an alternative offender (AO). The defence proposition will also be refined to account for the possibility of secondary transfer. The variables having a significant impact on the LR are identified taking advantage of a graphical probabilistic environment (using Bayesian Networks, BN), coupled with simulation techniques. The paper presents (a) a BN, based on previous work Taylor et al. [5]; (b) its parametrization based on the current literature that represents the current state of knowledge used to inform the conditional probability tables of the BN and; (c) the implementation of the simulation methods. Results show that, regardless of the DNA outcome obtained, the most impacting variable is the "DNA match probability" when the defence alleged that the POI has nothing to do with the incident. It means that, given the current state of knowledge, such cases can easily be interpreted considering activity level propositions as they would not require any further data acquisition. When secondary transfer is alleged under the defence's perspective, the LRs are generally much lower than for the previous case. The DNA match probability has less impact and variables associated with the donor will take the lead on the ranges observed on the LRs. Overall, once extraction and sampling efficiency have been set, the remaining variables that have an impact on the value of the evidence are the DNA quantity on hands and the background. With the most impacting variables so identified, it becomes manageable to direct further data acquisition if so required. Generally, the background that could be present on the knife handle, the environmental conditions are not critical due to their limited impact on the LR value. We note, however, that this identification of the significant variables depends on the obtained DNA results and this selection may be refined on a case by case basis. To allow one to explore all possibilities a dedicated Shiny application has been designed (https://lydie-samie.shinyapps.io/DNA_Activity/).
本文提出了一种方法,允许识别与 DNA 痕迹的转移、持续或恢复最相关的变量,这些变量对通过似然比 (LR) 测量的评估结果有最大影响。它基于一个案例情景,涉及从刀柄中回收的痕迹 DNA,检方指控嫌疑人刺伤了受害者,而辩方则声称嫌疑人与此事件无关,受害者是被另一名犯罪嫌疑人(AO)刺伤的。辩方的主张也将被细化,以考虑到二次转移的可能性。利用图形概率环境(使用贝叶斯网络,BN)和模拟技术,识别对 LR 有显著影响的变量。本文提出了:(a) 基于 Taylor 等人之前工作的 BN;(b) 基于当前文献的参数化,该文献代表了用于告知 BN 条件概率表的当前知识状态;(c) 模拟方法的实现。结果表明,无论获得的 DNA 结果如何,当辩方声称嫌疑人与此事件无关时,最具影响力的变量是“DNA 匹配概率”。这意味着,考虑到当前的知识状态,此类案件可以很容易地根据活动水平的主张进行解释,因为它们不需要进一步的数据采集。当辩方声称存在二次转移时,LR 通常要低得多。DNA 匹配概率的影响较小,与供体相关的变量将在观察到的 LR 范围内占据主导地位。总体而言,一旦确定了提取和采样效率,对证据价值有影响的剩余变量是手上的 DNA 数量和背景。通过确定最具影响力的变量,如果需要,可以对其进行进一步的数据采集。通常,刀柄上可能存在的背景以及环境条件不会对 LR 值产生影响,因此不是关键因素。然而,我们注意到,这些显著变量的识别取决于所获得的 DNA 结果,并且可以根据具体情况对这种选择进行细化。为了允许探索所有可能性,设计了一个专用的 Shiny 应用程序(https://lydie-samie.shinyapps.io/DNA_Activity/)。