Department of Orthopedic Physiotherapy, Güneysu Physical Therapy and Rehabilitation Faculty, Recep Tayyip Erdogan University, Rize, Turkey.
Department of Physiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey,
Cells Tissues Organs. 2020;209(1):64-74. doi: 10.1159/000507791. Epub 2020 Jun 19.
Osteoporosis is an important skeletal disease characterized by bone weakness and high risk of fracture in postmenopausal women. Tea consumption is known to play an important role in the prevention or alleviation of osteoporosis. However, the therapeutic effects of aqueous extracts of white tea (WT) have not been evaluated in osteoporosis rat models. The aim of this study was to investigate the potential anti-osteoporotic role of WT in ovariectomized (OVX) rats. WT was given orally at 0.5% w/v doses for 12 weeks in OVX rats. Biochemical parameters in blood samples, bone tartrate-resistant acid phosphatase (TRAP), C-terminal telopeptide of type 1 collagen (CTX) and estradiol levels were evaluated. Bone mineral density and bone mineral content values were measured in the left femur. In addition to histopathological examination, osteolcalcin, osteopontin and TUNEL levels were determined. OVX group data demonstrated that bone loss occurred by thinning of the metaphyseal growth plates of the femur. Similarly, the levels of TRAP and CTX, markers of osteoclastic activity, were found to be high concurrently with a decrease in femoral bone mineral density. In addition, increased osteolcalcin and osteopontin levels were present in the metaphyseal growth zones. On the other hand, while TRAP and CTX levels were suppressed in the OVX-WT group, bone mineral content increased. In ad-dition, TUNEL, osteocalcin and osteopontin positivity decreased in the right femoral metaphysis growth zones, proliferating zone and resting zone cells. These results showed that chronic WT consumption has a protective effect by reducing bone resorption in OVX-induced osteoporotic rats.
骨质疏松症是一种重要的骨骼疾病,其特征是绝经后妇女的骨骼脆弱和骨折风险高。茶的消费被认为在预防或缓解骨质疏松症方面发挥着重要作用。然而,白茶(WT)的水提取物在骨质疏松症大鼠模型中的治疗效果尚未得到评估。本研究旨在研究 WT 在去卵巢(OVX)大鼠中的潜在抗骨质疏松作用。WT 以 0.5% w/v 的剂量口服给予 OVX 大鼠 12 周。评估了血液样本中的生化参数、抗酒石酸酸性磷酸酶(TRAP)、I 型胶原 C 末端肽(CTX)和雌二醇水平。测量了左侧股骨的骨矿物质密度和骨矿物质含量值。除了组织病理学检查外,还测定了骨钙素、骨桥蛋白和 TUNEL 水平。OVX 组的数据表明,骨丢失是通过股骨骺板变薄发生的。同样,破骨细胞活性标志物 TRAP 和 CTX 的水平也很高,同时股骨骨矿物质密度降低。此外,骺板生长区的骨钙素和骨桥蛋白水平升高。另一方面,在 OVX-WT 组中,TRAP 和 CTX 水平受到抑制,而骨矿物质含量增加。此外,右股骨骺板生长区、增殖区和静止区细胞的 TUNEL、骨钙素和骨桥蛋白阳性减少。这些结果表明,慢性 WT 消耗通过减少 OVX 诱导的骨质疏松症大鼠的骨吸收具有保护作用。