Beder Melek, Yemenoglu Hatice, Bostan Semih Alperen, Kose Oğuz, Karakas Sibel Mataracı, Mercantepe Tolga, Yılmaz Adnan, Tumkaya Levent
Department of Periodontology, Faculty of Dentistry, Recep Tayyip Erdoğan University, Rize, 53020, Turkey.
Department of Biochemistry, Faculty of Medicine, Recep Tayyip Erdoğan University, Rize, Turkey.
BMC Oral Health. 2025 Jan 16;25(1):78. doi: 10.1186/s12903-025-05447-y.
To investigate the preventive efficacy of methylsulfonylmethane (MSM) on alveolar bone destruction in rats with periodontitis.
Twenty-four male Sprague-Dawley rats were randomly divided into three groups: control, experimental periodontitis (Ep), and Ep-MSM. Periodontitis was induced by placing 4.0 silk sutures in the subparamarginal position on the necks of the mandibular first molars and applying the suture for 5 weeks. The Ep-MSM group was given 500 mg/body weight/day MSM intraperitoneally for 35 days. At the end of the study, bilateral mandibular samples were taken. Periodontal bone loss was measured through histologic sections. Histomorphometric and immunohistochemical (receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG)) evaluations were performed on right mandibular tissue samples, and biochemical (interleukin (IL)-1 beta (β)/IL-10, malondialdehyde (MDA), glutathione (GSH), oxidative stress index (OSI)) evaluations were performed on left mandibular tissue samples.
No significant difference was found between the groups in IL-1β and IL-1β/IL-10 values (p > 0.05). A significant decrease in IL-10 levels was observed in the Ep-MSM and Ep groups compared with the control group (p < 0.05). MDA levels significantly increased in the Ep and Ep-MSM groups compared with the control group, and GSH levels significantly decreased in the Ep group compared with the other groups (p < 0.05). OSI values were significantly higher only in the Ep group (p < 0.05). RANKL levels showed a significant increase in the Ep group compared with the other groups. OPG levels were significantly increased only in the Ep-MSM group (p < 0.05).
The results of this study may suggest that MSM has preventive effects on alveolar bone loss and oxidative stress.
探讨甲磺酰甲烷（MSM）对牙周炎大鼠牙槽骨破坏的预防效果。
将24只雄性Sprague-Dawley大鼠随机分为三组：对照组、实验性牙周炎组（Ep）和Ep-MSM组。通过在下颌第一磨牙颈部龈下位置放置4.0丝线缝合5周来诱导牙周炎。Ep-MSM组腹腔注射500 mg/体重/天的MSM，持续35天。研究结束时，采集双侧下颌样本。通过组织学切片测量牙周骨丢失情况。对右侧下颌组织样本进行组织形态计量学和免疫组织化学（核因子κB受体活化因子配体（RANKL）、骨保护素（OPG））评估，对左侧下颌组织样本进行生化（白细胞介素（IL）-1β/IL-10、丙二醛（MDA）、谷胱甘肽（GSH）、氧化应激指数（OSI））评估。
各组间IL-1β和IL-1β/IL-10值无显著差异（p＞0.05）。与对照组相比，Ep-MSM组和Ep组IL-10水平显著降低（p＜0.05）。与对照组相比，Ep组和Ep-MSM组MDA水平显著升高，与其他组相比，Ep组GSH水平显著降低（p＜0.05）。仅Ep组的OSI值显著更高（p＜0.05）。与其他组相比，Ep组RANKL水平显著升高。仅Ep-MSM组OPG水平显著升高（p＜0.05）。
本研究结果可能提示MSM对牙槽骨丢失和氧化应激具有预防作用。
