Pokhil Sergiy I, Bondarenko Andriy V, Bocharova Tatiana V, Lepilina Kseniia M, Lytvynenko Marianna V, Gargin Vitaliy V
Mechnikov Institute of Microbiology and Immunology, Kharkiv, Ukraine.
Kharkiv National Medical University, Kharkiv, Ukraine.
Pol Merkur Lekarski. 2020 Jun 17;48(285):170-173.
Lifelong withdrawal from the donor population of those who have been diagnosed with babesiosis must be used for transmission prevention.
The aim of the study was a detection of Babesia antibodies level with the usage of experimental Babesia divergens whole-cell slide antigen and commercial B. microti immunofluorescence assay substrate slide (Fuller Laboratories, USA).
Experimental B. divergens whole-cell slide antigen in addition to commercial B. microti IFA substrate slide was used to create a diagnostic kit for serum Babesia antibodies level detecting, as well as for a babesiosis serodiagnosis clinical trial of different origins blood samples (patients with Lyme disease, rheumatoid arthritis and toxoplasmosis; human blood donors; cattle).
Antibodies to B. divergens (5.4%) and B. microti (2.3%) were detected with higher (p <0.05) frequency at Lyme disease patients (16.7%) than at blood donors (1.7%). Diagnostically significant IgG titres (= 1:128) were found in 13.3% of blood samples from Lyme disease patients and 1.7% from blood donors. Specific IgM were also found in 13.3% blood samples from Lyme disease patients. Among blood samples from Lyme disease patients, in which diagnostically significant titres of Babesia antibodies were detected (16.7%), 60% of them were represented by IgG and IgM (rA= 0.63), and in 40% only one of them reached diagnostically significant titre. Conclusions. Advantages of babesiosis IFA diagnostics.
Advantages of babesiosis IFA diagnostics are combined with its significant disadvantages (principle of evaluation, low sensitivity in the initial period of the disease, probability of false positives, absence of validated test systems and research protocols for B. divergens and B. divergens-like species).
对于已诊断为巴贝斯虫病的人，必须终生排除在献血人群之外以预防传播。
本研究的目的是使用实验性的分歧巴贝斯虫全细胞载玻片抗原和商业化的微小巴贝斯虫免疫荧光检测底物载玻片（美国富勒实验室）检测巴贝斯虫抗体水平。
除商业化的微小巴贝斯虫免疫荧光检测底物载玻片外，使用实验性的分歧巴贝斯虫全细胞载玻片抗原创建用于检测血清中巴贝斯虫抗体水平的诊断试剂盒，并对不同来源的血液样本（莱姆病、类风湿性关节炎和弓形虫病患者；人类献血者；牛）进行巴贝斯虫病血清学诊断临床试验。
莱姆病患者（16.7%）中检测到分歧巴贝斯虫抗体（5.4%）和微小巴贝斯虫抗体（2.3%）的频率高于献血者（1.7%）（p<0.05）。在莱姆病患者的13.3%血液样本和献血者的1.7%血液样本中发现具有诊断意义的IgG滴度（=1:128）。在莱姆病患者的13.3%血液样本中也发现了特异性IgM。在检测到具有诊断意义的巴贝斯虫抗体滴度的莱姆病患者血液样本中（16.7%），其中60%由IgG和IgM组成（rA = 0.63），40%中只有其中一种达到具有诊断意义的滴度。结论。巴贝斯虫病免疫荧光诊断的优势。
巴贝斯虫病免疫荧光诊断的优势与其显著缺点相结合（评估原则、疾病初期敏感性低、假阳性概率、缺乏针对分歧巴贝斯虫和类分歧巴贝斯虫物种的经过验证的检测系统和研究方案）。
