Li D, Yang X H, Zhang S H, Pan W Y, Zhou Y, Chen Z F, Wu R H
Fujian Provincial Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2020 Jun 10;41(6):946-951. doi: 10.3760/cma.j.cn112338-20190815-00599.
We isolated and identified the genotypes and molecular characteristics of the imported B3 measles virus (MeV) in Fujian province in 2018. Throat swab specimens were collected from clinically diagnosed measles patients and tested for viral RNA, using the real-time reverse transcription-polymerase chain reaction after the RNA extraction. Reverse transcription-polymerase chain reaction method was undertaken to amplify the 634 nucleotide acids of 3-terminal of the nucleoprotein gene. A phylogenetic tree was constructed and similarities in homology assessed. We successfully isolated and obtained two measles virus strains and eighteen viral nucleic acid sequences. The Fujian strains were clustered within the same genotype group of WHO genotype B3 reference strains. Compared to the major circulating measles strain genotype B3 in the world, two Fujian strains MV18-41 and MV18-42 showed 100.0 nucleic acid homology to HongKong.CHN/35.18 strain which was isolated from Hong Kong in 2018. The remaining 16 Fujian strains showed the highest homology (99.9) with the Mvs/Osaka.JPN/38.18/B3 strain isolated from Japan in 2018. Compared with other 23 WHO genotype reference strains, homology on both nucleotide and amino acid of the Fujian strain and the B1 genotype reference strain were the smallest, as 95.1-95.4 and 95.3, respectively. The differences of homology between the Fujian strain and H1 genotype reference strain were the largest, as 88.7-89.0 and 87.3, respectively. In addition, there were 13 mutation sites between the Fujian strain and the vaccine strain (Shanghai-191) at the 150 amino acid position of carboxy terminus on N protein, However, these sites did not cause functional changes in the protein region. In Fujian province, two strains of B3 genotype measles virus were obtained successfully, which were considered to be new genotype measles virus found in 2018. These findings showed it is necessary to strengthening the monitoring program on imported cases for better control and eliminate the measles virus.
我们分离并鉴定了2018年福建省输入性B3型麻疹病毒（MeV）的基因型和分子特征。从临床诊断的麻疹患者中采集咽拭子标本，提取RNA后，采用实时逆转录-聚合酶链反应检测病毒RNA。采用逆转录-聚合酶链反应方法扩增核蛋白基因3′端634个核苷酸。构建系统发育树并评估同源性相似性。我们成功分离并获得了两株麻疹病毒株和18条病毒核酸序列。福建毒株聚集在WHO基因型B3参考毒株的同一基因型组内。与世界上主要流行的麻疹毒株基因型B3相比，两株福建毒株MV18-41和MV18-42与2018年从香港分离的HongKong.CHN/35.18毒株核酸同源性为100.0%。其余16株福建毒株与2018年从日本分离的Mvs/Osaka.JPN/38.18/B3毒株同源性最高（99.9%）。与其他23株WHO基因型参考毒株相比，福建毒株与B1基因型参考毒株的核苷酸和氨基酸同源性最小，分别为95.1-95.4和95.3。福建毒株与H1基因型参考毒株的同源性差异最大，分别为88.7-89.0和87.3。此外，福建毒株与疫苗株（上海-191）在N蛋白羧基末端第150个氨基酸位置有13个突变位点，但这些位点未引起蛋白质区域功能变化。在福建省，成功获得了两株B3基因型麻疹病毒株，被认为是2018年发现的新型基因型麻疹病毒。这些发现表明，有必要加强输入病例监测程序，以更好地控制和消除麻疹病毒。
