Owens N F, Gingell D, Bailey J
Department of Anatomy, University College, London, UK.
J Cell Sci. 1988 Nov;91 ( Pt 3):367-77. doi: 10.1242/jcs.91.3.367.
Amoebae of the slime mould Dictyostelium discoideum form broad ultrathin cytoplasmic lamellae by a centripetal contractile process soon after they have spread on certain solid surfaces. We have investigated the surface requirements for initial triggering of this contact-mediated signalling system. The lamellar response is not normally evoked by glass, but is seen on glass covalently derivatized with paraffinic chains, as well as on glass covalently derivatized with amine groups and on glass bearing adsorbed polylysine. We have recorded the frequency of the lamellar response on these surfaces as a function of ionic strength and pH, and have measured the electrostatic potentials of the surfaces by the streaming potential method. Using these data we have concluded that the general trigger for the lamellar response is not a 'simple' physical or chemical property of the substrata: it is not dependent on specific chemical groups, degree of hydrophobicity, electrostatic potential, or charge density, taken as isolated factors. It seems likely that triggering is dependent on the overall energetics of cell-substratum interaction.
黏菌盘基网柄菌的变形虫在某些固体表面铺展后不久，通过向心收缩过程形成宽阔的超薄细胞质片层。我们研究了这种接触介导的信号系统初始触发的表面要求。正常情况下，玻璃不会引发片层反应，但在共价连接了石蜡链的玻璃上，以及在共价连接了胺基的玻璃和吸附了聚赖氨酸的玻璃上可以看到片层反应。我们记录了在这些表面上片层反应的频率与离子强度和pH的函数关系，并通过流动电位法测量了表面的静电势。利用这些数据，我们得出结论，片层反应的一般触发因素不是基质的“简单”物理或化学性质：它不依赖于特定的化学基团、疏水性程度、静电势或电荷密度等孤立因素。触发似乎取决于细胞 - 基质相互作用的整体能量学。
