Kistler J, Bullivant S
Department of Cellular, University of Auckland, New Zealand.
J Cell Sci. 1988 Nov;91 ( Pt 3):415-21. doi: 10.1242/jcs.91.3.415.
MIP and MP70 are putative gap junction components in the plasma membranes of the mammalian lens fibre cells. We show now that MP70 can be solubilized separately from MIP in mild detergent solutions, and that this treatment results in the dissociation of the fibre gap junctions. Solubilized MP70 was isolated as 16.9 S particles by velocity gradient centrifugation and in the electron microscope had the appearance of short double-membrane structures consistent with connexon-pairs. These observations open a new experimental avenue in which to characterize separately the two putative lens gap junction proteins structurally and functionally.
MIP和MP70是哺乳动物晶状体纤维细胞质膜中假定的间隙连接成分。我们现在表明，在温和的去污剂溶液中，MP70可以与MIP分开溶解，并且这种处理会导致纤维间隙连接的解离。通过速度梯度离心将溶解的MP70分离为16.9 S颗粒，在电子显微镜下呈现出与连接子对一致的短双膜结构外观。这些观察结果开辟了一条新的实验途径，可在其中分别从结构和功能上表征两种假定的晶状体间隙连接蛋白。
