Department of Research, Guangxi Medical University Cancer Hospital, Nanning 530021, China.
Department of Chemotherapy, Guangxi Medical University Cancer Hospital, Nanning 530021, China.
Dis Markers. 2020 May 20;2020:3159831. doi: 10.1155/2020/3159831. eCollection 2020.
The association of miR-28-5p with colon cancer remains to be elucidated. This study aimed to determine the clinical significance and prognostic value of miR-28-5p in colon cancer.
We retrospectively analyzed the data of miR-28-5p in colon adenocarcinoma data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), and the data was divided into cancer group and normal group, respectively. Forty colon cancer tissues and adjacent normal tissues were collected and tested by qRT-PCR methods. The difference of the miR-28-5p expression between colon cancer and normal tissues was compared. The clinical significance of miR-28-5p in colon cancer and the association with the survival were determined. The predictive value of miR-28-5p in clinical features was determined using receiver operating characteristic curve. The target genes of miR-28-5p were identified, and the functional of target genes was performed using bioinformatics analysis.
: The expression of miR-28-5p was increased in colon cancer tissues compared with normal controls ( = 0.037). The expression of miR-28-5p was significantly increased in tissues with distant metastases compared with that without distant metastases ( = 0.026). Patients with high expression of miR-28-5p have a shorter survival time than those with low expression ( = 0.004). Cox analysis showed that miR-28-5p was an independent predictor for the survival of patients ( = 0.014). Combination of miR-28-5p with TNM stage and clinical stage can improve the prognostic value for the patients ( < 0.05). miR-28-5p has a moderate predictive value in predicting the TNM stage and clinical stage (T stage: AUC = 0.515; N stage: AUC = 0.523, M stage: AUC = 0.572; clinical stage: AUC = 0.539). 711 potential target genes of miR-28-5p were screened; their function and pathways were identified.
: This study demonstrated that miR-28-5p was increased in colon cancer and can be an independent indicator for the overall survival in patients with colon cancer.
miR-28-5p 与结肠癌的关系尚待阐明。本研究旨在确定 miR-28-5p 在结肠癌中的临床意义和预后价值。
我们回顾性分析了来自癌症基因组图谱（TCGA）和基因表达综合数据库（GEO）的结肠癌 miR-28-5p 数据，并将数据分为癌症组和正常组。收集 40 例结肠癌组织和相邻正常组织，采用 qRT-PCR 方法进行检测。比较结肠癌与正常组织中 miR-28-5p 的表达差异。确定 miR-28-5p 在结肠癌中的临床意义及其与生存的关系。利用受试者工作特征曲线（ROC 曲线）确定 miR-28-5p 在临床特征中的预测价值。鉴定 miR-28-5p 的靶基因，并通过生物信息学分析研究靶基因的功能。
与正常对照组相比，结肠癌组织中 miR-28-5p 的表达升高（=0.037）。与无远处转移的组织相比，有远处转移的组织中 miR-28-5p 的表达显著升高（=0.026）。miR-28-5p 高表达的患者生存时间短于低表达的患者（=0.004）。Cox 分析表明，miR-28-5p 是患者生存的独立预测因子（=0.014）。miR-28-5p 与 TNM 分期和临床分期的联合可提高对患者的预后价值（<0.05）。miR-28-5p 对预测 TNM 分期和临床分期具有中等预测价值（T 分期：AUC=0.515；N 分期：AUC=0.523，M 分期：AUC=0.572；临床分期：AUC=0.539）。筛选出 miR-28-5p 的 711 个潜在靶基因，鉴定其功能和途径。
本研究表明，miR-28-5p 在结肠癌中升高，可作为结肠癌患者总生存期的独立指标。
