Department of Animal Science, Faculty of Agriculture, Tropical Feed Resources Research and Development Center (TROFREC), Khon Kaen University, Khon Kaen, Thailand.
Anim Biotechnol. 2022 Feb;33(1):116-127. doi: 10.1080/10495398.2020.1781146. Epub 2020 Jun 21.
This experiment was aimed to study the effect of sugarcane bagasse (SB) fermented with TH14, cellulase, and molasses on gas kinetics, nutrient digestibility, and ruminal fermentation patterns compared to rice straw (RS). A 2 × 2 × 2 (+1) factorial arrangement in a completely randomized design was used. Factor A was levels of TH14 at 0 and 0.05 g/kg fresh matter, factor B was levels of cellulase at 0 and 10 U/kg fresh matter, and factor C was levels of molasses at 0 and 5 g of substrate DM. The (+1) treatment referred to RS fermentation without additives. The results showed that kinetics of gas, gas production, and total volatile fatty acid were not different between RS and SB treatment. RS had significantly ( < 0.05) greater nutrient digestibility, ruminal pH, ammonia-nitrogen, and protozoa compared the control SB. Compared with control RS and SB, SB fermented with additives had greater ( < 0.05) gas from soluble fraction and rate constant of gas, dry matter and neutral detergent fiber digestibility, and ruminal propionate. In conclusion, SB fermented with TH14, cellulase, and molasses in combination promoted ruminal gas production, digestibility, and ruminal fermentation patterns.
本实验旨在研究甘蔗渣（SB）经 TH14、纤维素酶和糖蜜发酵后对气体动力学、养分消化率和瘤胃发酵模式的影响，并与稻草（RS）进行比较。采用完全随机设计的 2×2×2（+1）因子安排。因子 A 为 TH14 水平，分别为 0 和 0.05 g/kg 鲜物质，因子 B 为纤维素酶水平，分别为 0 和 10 U/kg 鲜物质，因子 C 为糖蜜水平，分别为 0 和 5 g 底物 DM。（+1）处理是指不加添加剂发酵的 RS。结果表明，RS 和 SB 处理之间的气体动力学、气体产生和总挥发性脂肪酸没有差异。RS 的养分消化率、瘤胃 pH 值、氨氮和原生动物显著（<0.05）高于对照 SB。与对照 RS 和 SB 相比，添加添加剂发酵的 SB 具有更高的（<0.05）可溶性部分气体、气体速率常数、干物质和中性洗涤纤维消化率以及瘤胃丙酸产量。总之，TH14、纤维素酶和糖蜜联合发酵 SB 可促进瘤胃气体产生、消化率和瘤胃发酵模式。
