Uotila Pyry M, Lemma Siria A, Haapasaari Kirsi-Maria, Porvari Katja, Skarp Sini, Soini Ylermi, Jantunen Esa, Turpeenniemi-Hujanen Taina, Kuittinen Outi
Department of Oncology and Radiotherapy, Oulu University Hospital and University of Oulu, Oulu, Finland.
Medical Research Center and Unit of Translational Medicine, Oulu University Hospital and University of Oulu, Oulu, Finland.
Hematology. 2020 Dec;25(1):241-246. doi: 10.1080/16078454.2020.1780754.
To investigate the protein expression of the epithelial-mesenchymal transition-inducing transcription factors (TFs) Twist, ZEB1 and Slug in peripheral T-cell lymphomas (PTCL) and their correlation with clinical parameters. The expression of these TFs was studied in 53 diagnostic biopsy specimens of several different PTCL subtypes with immunohistochemistry. Patient data were retrospectively collected from patient records and a statistical analysis was performed. All three TFs were widely expressed. ZEB1 and Slug had correlations with clinical outcome. In all PTCL cases, high nuclear ZEB1 percentage correlated with a favorable progression-free survival (PFS) (3-year PFS: 70% vs. 34%; = 0.010) and strong nuclear Slug intensity correlated with an unfavorable PFS (3-year PFS: 17% vs. 62%; = 0.036). The correlations between PFS and ZEB1 or Slug protein expression have not previously been established in PTCLs. The impact of ZEB1 and Slug expression on prognosis differed from our findings in DLBCL and the impact of ZEB1 expression was in line with current studies on mycosis fungoides and sézary syndrome. The findings may be explained by the roles these TFs play in hematopoiesis. ZEB1 and Slug may have potential clinical value for evaluating prognosis in PTCLs. The study size was small and heterogenous, and larger studies are warranted.
研究上皮-间质转化诱导转录因子（TFs）Twist、ZEB1和Slug在外周T细胞淋巴瘤（PTCL）中的蛋白表达及其与临床参数的相关性。采用免疫组织化学方法，在53例不同PTCL亚型的诊断性活检标本中研究了这些TFs的表达情况。从患者记录中回顾性收集患者数据并进行统计分析。所有三种TFs均广泛表达。ZEB1和Slug与临床结局相关。在所有PTCL病例中，高核ZEB1百分比与较好的无进展生存期（PFS）相关（3年PFS：70%对34%；P = 0.010），而强核Slug强度与不良PFS相关（3年PFS：17%对62%；P = 0.036）。PTCL中PFS与ZEB1或Slug蛋白表达之间的相关性此前尚未确立。ZEB1和Slug表达对预后的影响与我们在弥漫性大B细胞淋巴瘤（DLBCL）中的发现不同，ZEB1表达的影响与目前关于蕈样肉芽肿和塞扎里综合征的研究一致。这些发现可能由这些TFs在造血过程中所起的作用来解释。ZEB1和Slug在评估PTCL预后方面可能具有潜在的临床价值。本研究样本量小且具有异质性，需要开展更大规模的研究。
