Stewart C C, Stevenson A P, Habbersett R C
Los Alamos National Laboratory, New Mexico 87545.
Int J Radiat Biol Relat Stud Phys Chem Med. 1988 Jan;53(1):77-87. doi: 10.1080/09553008814550441.
A culture system was used to evaluate the radiosensitivity of CD4+ and CD8+ T cells, Leu 19+ cells, and B cells obtained from normal adult males. Unstimulated CD8+ lymphocytes (D0 = 55 cGy) were twice as radiosensitive as CD4+ cells (D0 = 115 cGy). B cells had an intermediate radiosensitivity (D0 = 100 cGy). Leu 19+ cells were much more radioresistant and expressed a D0 of 550 cGy. When lymphoid cells were irradiated 1 or 4 days before phytohemagglutinin (PHA) stimulation, they were more radiosensitive than if they were first stimulated with PHA and then irradiated. When lymphoid cells were irradiated 1 h after PHA stimulation each lymphocyte subset was characterized by an increase in the D0 to 150 cGy for B cells to 290 cGy for CD4+ cells, and to 240 cGy for CD8+ cells. In contrast, Leu 19+ cells exhibited a decrease in their D0 to 290 cGy after they were stimulated by PHA.
采用一种培养系统来评估从正常成年男性获取的CD4+和CD8+ T细胞、Leu 19+细胞及B细胞的放射敏感性。未受刺激的CD8+淋巴细胞（D0 = 55 cGy）的放射敏感性是CD4+细胞（D0 = 115 cGy）的两倍。B细胞具有中等放射敏感性（D0 = 100 cGy）。Leu 19+细胞对辐射的抗性更强，其D0为550 cGy。当淋巴细胞在植物血凝素（PHA）刺激前1天或4天受到照射时，它们比先经PHA刺激再照射时更具放射敏感性。当淋巴细胞在PHA刺激后1小时受到照射时，每个淋巴细胞亚群的特征是D0增加，B细胞增至150 cGy，CD4+细胞增至290 cGy，CD8+细胞增至240 cGy。相比之下，Leu 19+细胞在受到PHA刺激后其D0降至290 cGy。
