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含二硫键肽段的片段合成

Fragment synthesis of disulfide-containing peptides.

作者信息

Dai Yuxuan, Yue Na, Huang Wenlong, Qian Hai

机构信息

Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, PR China.

Jiangsu Key Laboratory of Drug Discovery for Metabolic Disease, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, PR China.

出版信息

MethodsX. 2020 Jun 4;7:100945. doi: 10.1016/j.mex.2020.100945. eCollection 2020.

DOI:10.1016/j.mex.2020.100945
PMID:32577409
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7303597/
Abstract

A new strategy for solid phase peptide synthesis in the fragment synthesis based on the use of 2-Cl-trityl resin as carrier to obtain protected peptide-resin and Rink Amide Resin, HOBT/HBTU or PyBOP/DIPEA or HATU/DIPEA as the coupling method is described. The highlights of this method are:•Simple.•Low cost.•Solid phase based.

摘要

描述了一种基于使用2-氯三苯甲基树脂作为载体以获得受保护的肽树脂的片段合成中固相肽合成的新策略，以及使用瑞诺酰胺树脂、HOBT/HBTU或PyBOP/DIPEA或HATU/DIPEA作为偶联方法。该方法的亮点包括：•简单。•低成本。•基于固相。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/db1ec6824b4f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/3e0183b979fe/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/cf5d158d43bc/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/06b6736c3afd/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/e785f8c1e2bc/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/f51a9195e3fa/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/db1ec6824b4f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/3e0183b979fe/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/cf5d158d43bc/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/06b6736c3afd/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/e785f8c1e2bc/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/f51a9195e3fa/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e99e/7303597/db1ec6824b4f/gr5.jpg

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本文引用的文献

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Peptide conjugates of lactoferricin analogues and antimicrobials-Design, chemical synthesis, and evaluation of antimicrobial activity and mammalian cytotoxicity.乳铁蛋白类似物和抗菌肽缀合物的设计、化学合成及抗菌活性和哺乳动物细胞毒性评价。
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固相氮杂环丁烷二烯加成肽 Diels-Alder 化学用于氮杂哌啶酮残基合成以研究肽构象。
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Solid phase synthesis.固相合成
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