Hadar E J, Ershler W B, Kreisle R A, Ho S P, Volk M J, Klopp R G
Department of Medicine, University of Wisconsin, Madison 53706.
Cancer Immunol Immunother. 1988;26(1):31-4. doi: 10.1007/BF00199844.
Lymphocyte-induced angiogenesis factor (LIA) is a product of T lymphocytes which has been shown to stimulate new vessel formation. Because immune senescence most profoundly affects T lymphocyte functions, we suspected that LIA production would decline with age. An assay for angiogenesis stimulated by allogeneic reaction was performed by injecting spleen cells from young or old donor mice into the skin of irradiated allogeneic recipient mice. The spleen cells from young mice induced a significantly greater number of vessels than did cells from older mice. In additional experiments, spleen cells from young and old animals were treated with a monoclonal antibody GK 1.5) directed at the L3T4 antigen on murine T helper lymphocytes. Such treatment significantly reduced the new vessel formation induced by young lymphocytes but had no effect on that induced by lymphocytes from old animals. Studies employing indirect immunofluorescence demonstrated that the proportion of L3T4+ cells in the mononuclear fraction of splenocytes was nearly identical in both young and old mice. From these investigations we can conclude that (1) L3T4+ lymphocytes are responsible for LIA production, and (2) production, like that of other T lymphokines, declines with age.
淋巴细胞诱导的血管生成因子（LIA）是T淋巴细胞的一种产物，已被证明能刺激新血管形成。由于免疫衰老对T淋巴细胞功能的影响最为显著，我们推测LIA的产生会随着年龄的增长而下降。通过将年轻或年老供体小鼠的脾细胞注射到经辐照的同种异体受体小鼠的皮肤中，进行了同种异体反应刺激的血管生成测定。年轻小鼠的脾细胞诱导生成的血管数量明显多于年老小鼠的细胞。在另外的实验中，用针对小鼠T辅助淋巴细胞上L3T4抗原的单克隆抗体（GK 1.5）处理年轻和年老动物的脾细胞。这种处理显著减少了年轻淋巴细胞诱导的新血管形成，但对年老动物淋巴细胞诱导的新血管形成没有影响。采用间接免疫荧光的研究表明，年轻和年老小鼠脾细胞单核部分中L3T4+细胞的比例几乎相同。从这些研究中我们可以得出结论：（1）L3T4+淋巴细胞负责LIA的产生，（2）与其他T淋巴细胞因子一样，LIA的产生会随着年龄的增长而下降。
