Hanau D, Fabre M, Schmitt D A, Garaud J C, Pauly G, Cazenave J P
INSERM U.311, Université Louis Pasteur, France.
J Invest Dermatol. 1988 Mar;90(3):298-304. doi: 10.1111/1523-1747.ep12456083.
We have previously demonstrated by immunogold labeling techniques that the T6 surface antigen of living human Langerhans cells (LC) of the skin is internalized by a process of receptor-mediated endocytosis (RME). Receptor-mediated endocytosis induced by the binding of the monoclonal anti-T6 antibody BL6 involves coated pits, coated vesicles, endosomes, the smooth endoplasmic reticulum, and lysosomes. After RME, gold-labeled Birbeck granules (BGs) appear in the cell center. In the present study, by modifying the experimental conditions but retaining the presence of gold-labeled BL6, we have observed (in addition to the above-mentioned gold-labeled organelles) images that appear to correspond to BG-like structures. These structures, like the intracytoplasmic BGs, are pentalaminar and gold-labeled, but they differ from the intracytoplasmic BGs by their attachment to the cell membrane, their length, the frequent presence of a coat, and by prolongation by one or more coated pits. Birbeck granule-like structures seem to result, at the ultrastructural level, from receptor ligand interactions and from modifications of the LC shape.
我们先前通过免疫金标记技术证明，皮肤中活的人类朗格汉斯细胞（LC）的T6表面抗原是通过受体介导的内吞作用（RME）内化的。由单克隆抗T6抗体BL6结合诱导的受体介导的内吞作用涉及被膜小窝、被膜小泡、内体、滑面内质网和溶酶体。在RME之后，金标记的伯贝克颗粒（BGs）出现在细胞中心。在本研究中，通过改变实验条件但保留金标记的BL6的存在，我们观察到（除上述金标记的细胞器外）一些似乎与BG样结构相对应的图像。这些结构与胞质内的BGs一样，是五片层的且被金标记，但它们与胞质内的BGs不同，在于它们附着于细胞膜、它们的长度、频繁出现的被膜以及由一个或多个被膜小窝延伸而来。在超微结构水平上，伯贝克颗粒样结构似乎是由受体配体相互作用以及LC形状的改变导致的。
