Orexin 受体阻断对大鼠海马齿状回神经元磷脂酶 C-β3 水平影响的免疫组织化学研究。
An immunohistochemical study of the effects of orexin receptor blockade on phospholipase C-β3 level in rat hippocampal dentate gyrus neurons.
机构信息
Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Lab Medical Sciences, Allied Medical College, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Biotech Histochem. 2021 Apr;96(3):191-196. doi: 10.1080/10520295.2020.1778088. Epub 2020 Jun 25.
Orexin-A (hypocretin-1) is a neuropeptide that is produced in the lateral hypothalamic area (LHA) and promotes widespread cortical activation. We investigated the effect of SB-334867, a selective orexin receptor 1 (OXR1) antagonist, on phospholipase C-β3 (PLCβ3) level using slices of rat hippocampus preparations and immunohistochemistry. We used three Wistar rats in each of three groups. The control group was untreated rats and SB vehicle and SB groups received SB vehicle and 10 mg/kg SB-334867 daily from postnatal day (PND) 12 to PND30, respectively. We found that the orexin receptor antagonist decreased the PLCβ3 level in the inner and outer blades of dentate gyrus (DG) compared to SB vehicle treated rats. Orexin may increase the PLCβ3 level in most regions of the rat hippocampus.
食欲素-A（下丘脑泌素-1）是一种神经肽，在外侧下丘脑区域（LHA）产生，促进广泛的皮质激活。我们使用大鼠海马切片和免疫组织化学研究了选择性食欲素受体 1（OXR1）拮抗剂 SB-334867 对磷酯酶 C-β3（PLCβ3）水平的影响。我们在三个组中各使用了三只 Wistar 大鼠。对照组为未处理大鼠和 SB 载体，SB 组分别从出生后第 12 天（PND）至第 30 天（PND）每天接受 SB 载体和 10mg/kg SB-334867。我们发现与接受 SB 载体处理的大鼠相比，食欲素受体拮抗剂降低了齿状回（DG）内叶和外叶的 PLCβ3 水平。食欲素可能会增加大鼠海马体大多数区域的 PLCβ3 水平。
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