Department of Biophysics, Basic Medical Sciences, Panjab University, Chandigarh, India.
Int J Vitam Nutr Res. 2021 Sep;91(5-6):396-410. doi: 10.1024/0300-9831/a000660. Epub 2020 Jun 25.
The present study aimed to examine the chemoprotective effect of Hydroethanolic leaves extract (HEMKLE) on murine skin carcinogenesis model. For the study, male LACA mice divided into four groups (n = 15 per group). Group I (Control), Group II (DMBA/TPA), Group III (HEMKLE), and Group IV (HEMKLE + DMBA/TPA). Skin tumors were induced in Group II (DMBA/TPA) and Group IV (HEMKLE + DMBA/TPA) by topical application of 7, 12 dimethylbenz[a]anthracene (DMBA) [500 nmol/100 μL of acetone, twice a week for two weeks] and 12-O-tetradecanoyl phorbol-13-acetate (TPA) [1.7 nmol/100 μL of acetone, twice a week for eighteen weeks] and HEMKLE (200 mg/kg b. w.) was administered orally (instilled by oral gavage). The chemoprotective response of HEMKLE was evident by inhibition in tumor incidence, mean tumor volume, mean tumor burden, total number of tumors, and tumor size in Group IV (HEMKLE + DMBA/TPA) when compared to Group II (DMBA/TPA). HEMKLE administration also decreased the reactive oxygen species (ROS) and lipid peroxidation (LPO) levels and increased the antioxidants enzyme activities in Group IV (HEMKLE + DMBA/TPA) when compared to Group II (DMBA/TPA) that suggests its antioxidant potential. HEMKLE administration also increased the mRNA and protein expression of caspase-9 and caspase-3 and decreased the mRNA and protein expression of Bcl-2 in Group IV (HEMKLE + DMBA/TPA) when compared to Group II (DMBA/TPA) that suggest its apoptosis-inducing effect on DMBA/TPA induced skin carcinogenesis.
本研究旨在探讨水醇提 Leaves 提取物 (HEMKLE) 对小鼠皮肤癌变模型的化学预防作用。在这项研究中，雄性 LACA 小鼠分为四组（每组 15 只）。第 I 组（对照组）、第 II 组（DMBA/TPA）、第 III 组（HEMKLE）和第 IV 组（HEMKLE+DMBA/TPA）。第 II 组（DMBA/TPA）和第 IV 组（HEMKLE+DMBA/TPA）通过局部涂抹 7,12-二甲基苯并[a]蒽（DMBA）[500nmol/100μL 丙酮，每周两次，共两周]和 12-O-十四烷酰佛波醇-13-乙酸酯（TPA）[1.7nmol/100μL 丙酮，每周两次，共十八周]诱导皮肤肿瘤，同时给予 HEMKLE（200mg/kg b.w.）口服（灌胃）。与第 II 组（DMBA/TPA）相比，第 IV 组（HEMKLE+DMBA/TPA）的肿瘤发生率、平均肿瘤体积、平均肿瘤负荷、肿瘤总数和肿瘤大小均有所抑制，表明 HEMKLE 具有化学预防作用。与第 II 组（DMBA/TPA）相比，HEMKLE 给药还降低了第 IV 组（HEMKLE+DMBA/TPA）的活性氧（ROS）和脂质过氧化（LPO）水平，并增加了抗氧化酶活性，表明其具有抗氧化潜力。与第 II 组（DMBA/TPA）相比，HEMKLE 给药还增加了第 IV 组（HEMKLE+DMBA/TPA）的 caspase-9 和 caspase-3 mRNA 和蛋白表达，降低了 Bcl-2 的 mRNA 和蛋白表达，表明其对 DMBA/TPA 诱导的皮肤癌变具有诱导细胞凋亡的作用。
