Jensen Camilla, Li Heng, Vestergaard Martin, Dalsgaard Anders, Frees Dorte, Leisner Jørgen J
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore.
Front Microbiol. 2020 Jun 3;11:1007. doi: 10.3389/fmicb.2020.01007. eCollection 2020.
Nisin is applied as a food preservative in processed foods and has the potential to be used synergistically with antibiotics for treatment of patients infected by antibiotic-resistant bacteria, such as methicillin-resistant . The present study explores the antimicrobial effect of nisin on viability and membrane integrity and, for the first time, used super-resolution microscopy to study morphological changes induced in cells exposed to nisin. The exposure of to nisin caused membrane depolarization and rapid killing. Super-resolution structured-illumination microscopy and transmission electron microscopy confirmed that nisin damages the cellular membrane and causes lysis of cells. Strikingly, condensation of chromosomal DNA was observed in all cells exposed to nisin, a phenotype not previously reported for this compound. Moreover, cells exposed to nisin were significantly smaller than non-exposed cells indicating the emergence of cell shrinkage. The strong association of DNA condensation with nisin exposure indicates that nisin interferes with chromosome replication or segregation in .
乳链菌肽作为一种食品防腐剂应用于加工食品中，并且有可能与抗生素协同用于治疗由耐抗生素细菌感染的患者，如耐甲氧西林细菌。本研究探讨了乳链菌肽对细胞活力和膜完整性的抗菌作用，并且首次使用超分辨率显微镜研究暴露于乳链菌肽的细胞中诱导的形态变化。将细胞暴露于乳链菌肽会导致膜去极化和快速杀伤。超分辨率结构光照明显微镜和透射电子显微镜证实，乳链菌肽会损害细胞膜并导致细胞裂解。引人注目的是，在所有暴露于乳链菌肽的细胞中都观察到染色体DNA凝聚，这是该化合物以前未报道过的一种表型。此外，暴露于乳链菌肽的细胞明显小于未暴露的细胞，表明出现了细胞收缩。DNA凝聚与乳链菌肽暴露的强烈关联表明，乳链菌肽会干扰细胞中的染色体复制或分离。
