Imoto M, Yamashita T, Sawa T, Kurasawa S, Naganawa H, Takeuchi T, Bao-quan Z, Umezawa K
Institute of Microbial Chemistry, Tokyo, Japan.
FEBS Lett. 1988 Mar 28;230(1-2):43-6. doi: 10.1016/0014-5793(88)80638-0.
Psi-tectorigenin, an isoflavonoid, was isolated from a culture filtrate of actinomycetes as an inhibitor of epidermal growth factor-induced phosphatidylinositol turnover in cultured A431 cells. It inhibited phosphatidylinositol turnover with an IC50 of about 1 microgram/ml; thus, its inhibitory activity was 6-times stronger than that of genistein or orobol. When added to cultured A431 cells psi-tectorigenin inhibited phosphatidylinositol turnover without inhibiting epidermal growth factor receptor tyrosine protein kinase. Thus, psi-tectorigenin is a specific inhibitor of phosphatidylinositol turnover and may be a useful tool for the functional analysis of phosphatidylinositol turnover.
次甲基伪紫罗兰酮，一种异黄酮，从放线菌的培养滤液中分离出来，作为培养的A431细胞中表皮生长因子诱导的磷脂酰肌醇周转的抑制剂。它以约1微克/毫升的IC50抑制磷脂酰肌醇周转；因此，其抑制活性比染料木黄酮或芒柄花素强6倍。当添加到培养的A431细胞中时，次甲基伪紫罗兰酮抑制磷脂酰肌醇周转而不抑制表皮生长因子受体酪氨酸蛋白激酶。因此，次甲基伪紫罗兰酮是磷脂酰肌醇周转的特异性抑制剂，可能是磷脂酰肌醇周转功能分析的有用工具。
