Sohan Forooshan Moghadam Atefeh, Ataei Mitra, Arabzadeh Ghazaleh, Falahati Kowsar, Roshani Fatemeh, Sanati Mohammad Hossein
Department of Biology, Nourdanesh Institute of Higher Education, Meymeh, Isfahan, Iran.
Medical Genetic Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Rep Biochem Mol Biol. 2020 Jan;8(4):429-437.
In multiple sclerosis (MS), the immune system acts against myelin lesions of the central nervous system, destroying neuronal fibers resulting in signal transmission disturbances in the nervous system. MicroRNAs play important roles in the post-transcriptional regulation of gene expression and in the regulation of disease activity and its response to treatment. The goal of this study was to determine the role of by comparing its expression in MS patients and healthy subjects.
RNA was isolated from blood samples of 32 MS patients and 32 healthy individuals, and miR-18a-5p expression was determined by real-time polymerase chain reaction (real-time PCR).
expression was significantly less in MS patients than in healthy subjects.
The reduction of expression may be via pathway signaling. Altered signaling plays an important role in MS pathogenesis and the expression profile in blood cells can be described as a prognostic biomarker and identifier of high-risk individuals in MS.
在多发性硬化症（MS）中，免疫系统攻击中枢神经系统的髓鞘病变，破坏神经元纤维，导致神经系统信号传递紊乱。微小RNA在基因表达的转录后调控以及疾病活动及其对治疗的反应调节中发挥重要作用。本研究的目的是通过比较其在MS患者和健康受试者中的表达来确定[具体物质]的作用。
从32例MS患者和32例健康个体的血液样本中分离RNA，并通过实时聚合酶链反应（实时PCR）测定miR-18a-5p的表达。
[具体物质]在MS患者中的表达明显低于健康受试者。
[具体物质]表达的降低可能通过[具体]信号通路。信号通路改变在MS发病机制中起重要作用，血细胞中的[具体物质]表达谱可被描述为MS高危个体的预后生物标志物和识别指标。
