Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France.
Departamento Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos. Facultad de Veterinaria. Universidad Cardenal Herrera-CEU, CEU Universities. Valencia, Spain.
J Infect Dis. 2022 Mar 15;225(6):1005-1010. doi: 10.1093/infdis/jiaa367.
The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture, including the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring L. monocytogenes cytoplasmic access. Furthermore, we showed that Taok2 recruitment to L. monocytogenes vacuoles requires the presence of pore-forming toxin listeriolysin O. Overall, our study identified the first set of host factors modulating L. monocytogenes vacuolar rupture and cytoplasmic access in epithelial cells.
细菌病原体李斯特菌属侵入宿主细胞，破坏内化液泡，并进入细胞质进行复制。高内涵小干扰 RNA（siRNA）显微镜筛选使我们能够鉴定参与李斯特菌属液泡破裂的上皮细胞因子，包括丝氨酸/苏氨酸激酶 Taok2。使用特定药物抑制激酶活性验证了 Taok2 在促进李斯特菌属细胞质进入方面的作用。此外，我们还表明 Taok2 募集到李斯特菌属液泡需要形成孔的毒素李斯特菌溶血素 O 的存在。总的来说，我们的研究鉴定了一组调节上皮细胞中李斯特菌属液泡破裂和细胞质进入的宿主因子。
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