Fenton M J, Vermeulen M W, Clark B D, Webb A C, Auron P E
Harvard University-Massachusetts Institute of Technology, Division of Health Sciences and Technology, Cambridge 02139.
J Immunol. 1988 Apr 1;140(7):2267-73.
We have previously reported that the pro-IL-1 beta gene is transiently expressed in THP-1 human monocytic leukemia cells after stimulation with bacterial LPS. Herein we show differential pro-IL-1 beta gene transcription in the same cell type using two distinct stimuli. This transcriptional difference is also reflected at the level of intracellular IL-1 beta protein production. In contrast to LPS, a phorbol ester (PMA) induces a stable, non-transient population of mRNA. Furthermore, each induction pathway is operational under conditions where the other is inhibited, suggesting functional independence. Evidence is presented for post-transcriptional regulation of the two responses in THP-1 cells at the level of mRNA stability. We also demonstrate a similar dualistic response in primary monocyte-derived human macrophages as well as the human myelocytic cell line HL-60.
我们之前曾报道，在用细菌脂多糖刺激后，白细胞介素-1β前体（pro-IL-1β）基因在THP-1人单核细胞白血病细胞中短暂表达。在此，我们展示了使用两种不同刺激物时，同一细胞类型中pro-IL-1β基因转录的差异。这种转录差异也反映在细胞内白细胞介素-1β蛋白产生的水平上。与脂多糖不同，佛波酯（PMA）诱导产生稳定的、非短暂的mRNA群体。此外，每种诱导途径在另一种途径被抑制的条件下仍可发挥作用，表明其功能具有独立性。有证据表明，在THP-1细胞中，两种反应在mRNA稳定性水平上受到转录后调控。我们还在原代单核细胞衍生的人巨噬细胞以及人髓细胞系HL-60中证明了类似的二元反应。
