Atef Maryam, Ojagh Seyed Mahdi, Latifi Ali Mohammad, Esmaeili Mina, Udenigwe Chibuike C
Department of Seafood Science and Technology, Faculty of Fisheries and Environmental Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
School of Nutrition Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, ON, Canada.
J Food Biochem. 2020 Aug;44(8):e13256. doi: 10.1111/jfbc.13256. Epub 2020 Jun 24.
The potential use of sturgeon fish skin waste (Huso huso), an Iranian major sturgeon species, as a rich source for collagen extraction was evaluated. Yields of ASC and PSC obtained by acidic and enzymatic extractions were 9.98% and 9.08% (based on wet weight), respectively. SDS-PAGE profiles of both collagens led to classification of the proteins as type I with two different α chains (α and α ). Scanning electron microscopy (SEM) of the collagen sponges indicated dense sheet-like film linked by random-coiled filaments. Glycine was the most predominant amino acid, and the imino acids contents were 21.14% and 21.58% for ASC and PSC, respectively. Fourier-transform infrared spectra (FTIR) confirmed that pepsin digestion did not disrupt PSC triple helical structure. Denaturation and melting temperatures of ASC and PSC were 29.34°C, 92.03°C, and 29.89°C, 88.93°C, respectively. Thus, the sturgeon fish skin waste could serve as an alternative collagenous source for biomedical materials, food, and pharmaceutical applications. PRACTICAL APPLICATIONS: Beluga (Huso huso) is one of the most important sturgeon fish on the Caspian Sea and aquaculture industries. With the exception of the meat and caviar, wastes generated after their processing are usually discarded. Skin and cartilage of sturgeon fish are the by-products of the processing, and they are often discarded as waste or used for low-value purposes, although they are a good source for production of collagen-based biomaterials. Collagen type I is the most abundant collagen in the skin and this work reports the sturgeon fish skin as an important collagen resource with potential for use in the food, biomedical, and cosmetic industries.
对伊朗主要鲟鱼品种——俄罗斯鲟鱼的鱼皮废料作为胶原蛋白提取丰富来源的潜在用途进行了评估。通过酸性和酶促提取获得的酸溶性胶原蛋白（ASC）和胃蛋白酶溶性胶原蛋白（PSC）的产量分别为9.98%和9.08%（基于湿重）。两种胶原蛋白的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）图谱将这些蛋白质归类为含有两条不同α链（α1和α2）的I型胶原蛋白。胶原蛋白海绵的扫描电子显微镜（SEM）显示，致密的片状薄膜由无规卷曲的细丝连接。甘氨酸是最主要的氨基酸，ASC和PSC的亚氨基酸含量分别为21.14%和21.58%。傅里叶变换红外光谱（FTIR）证实，胃蛋白酶消化不会破坏PSC的三螺旋结构。ASC和PSC的变性温度和熔点分别为29.34℃、92.03℃和29.89℃、88.93℃。因此，鲟鱼鱼皮废料可作为生物医学材料、食品和制药应用的替代胶原来源。实际应用：大白鲸（俄罗斯鲟）是里海和水产养殖业最重要的鲟鱼之一。除了鱼肉和鱼子酱外，加工后产生的废料通常被丢弃。鲟鱼的皮肤和软骨是加工的副产品，尽管它们是生产基于胶原蛋白的生物材料的良好来源，但它们经常被作为废物丢弃或用于低价值用途。I型胶原蛋白是皮肤中最丰富的胶原蛋白，这项工作报道了鲟鱼鱼皮是一种重要的胶原蛋白资源，具有在食品、生物医学和化妆品行业应用的潜力。
