Kurokawa R, Kyakumoto S, Ota M
Department of Biochemistry, Iwate Medical University School of Dentistry, Japan.
J Endocrinol. 1988 Mar;116(3):451-5. doi: 10.1677/joe.0.1160451.
The treatment of a human submandibular gland adenocarcinoma cell line (HSG cell line) for 48 h with triamcinolone acetonide (TA; 1-100 nmol/l) reduced the secretion of epidermal growth factor (EGF) in a closely related manner to a maximum of 66%. The reduction in the level of EGF secreted resulted in the suppression of DNA synthesis in the HSG cells to a similar extent. When the cells were incubated with TA and exogenous human EGF (hEGF), DNA synthesis was 1.7-fold higher than that without added hEGF. The removal of EGF by the addition of hEGF antibody reduced DNA synthesis in HSG cell cultures to the same extent as did TA. These results suggest that the growth inhibition of HSG cells by TA is due to the reduction in the amount of EGF secreted.
用曲安奈德（TA；1 - 100 nmol/l）处理人下颌下腺腺癌细胞系（HSG细胞系）48小时，以密切相关的方式使表皮生长因子（EGF）的分泌最多减少66%。分泌的EGF水平降低导致HSG细胞中的DNA合成受到类似程度的抑制。当细胞与TA和外源性人EGF（hEGF）一起孵育时，DNA合成比未添加hEGF时高1.7倍。添加hEGF抗体去除EGF使HSG细胞培养物中的DNA合成减少程度与TA相同。这些结果表明，TA对HSG细胞的生长抑制是由于分泌的EGF量减少所致。
