Department of Natural Products Research, Dr. Koirala Research Institute for Biotechnology and Biodiversity, Kathmandu 44600, Nepal.
Institute of Agricultural Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore-54590, Pakistan.
Cell Mol Biol (Noisy-le-grand). 2020 Jun 25;66(4):1-7.
Vitex negundo (VN) is a widely used plant in folk medicine, namely for the treatment of jaundice, wounds, body ache, toothache, asthma, eye pain, and migraine. These effects have been increasingly attributed to its chemical composition. Here, we assessed the VN chemical and nutritional composition and biological activity, with particular emphasis on antioxidant and antimicrobial effects. VN methanol and hexane extracts revealed the presence of important phytochemical, such as terpenoids, polyphenol, steroids, saponins, phenolic compound and flavonoids. Total phenolic content of VN methanol extract from bark was 1082.473 mg/g GAE and that of leaves was 1382.984 mg/g GAE. The total flavonoids content in VN methanol extract from VN bark was 127.744 mg/g QE and that of leaves was 123.776 mg/g QE. VN methanol extract from bark exhibited high antioxidant effects (IC50=38.47 ppm). The content (%) of ash, moisture, crude fiber, crude protein and fat in VN leaves was, respectively, 7.86%, 18.35%, 6.52%, 9.687% and 6.19%. VN leaves methanol extract revealed antimicrobial activity against Bacillus subtilis and Staphylococcus aureus, with inhibition halos being, respectively, 13 mm and 14 mm, and the MBC values were found to be 1.562 mg/mL and 6.25 mg/mL, respectively. Similarly, VN bark methanol extract led to inhibition halos of 18 mm and 15 mm for B. subtilis and S. aureus, respectively, and the MBC values were found to be 2.372 mg/mL and 0.245 mg/mL. GC-MS analysis of the VN bark methanol extract revealed that monoolein was the major compounds, with an area of 0.57%. Thus, our results encourage the potential use of VN as a medicinal product, with high protein contents, and prominent antioxidant and anti-bacterial effects.
荆条（VN）是一种广泛应用于民间医学的植物，用于治疗黄疸、伤口、身体疼痛、牙痛、哮喘、眼痛和偏头痛。这些作用越来越归因于其化学成分。在这里，我们评估了 VN 的化学成分和营养成分以及生物活性，特别强调抗氧化和抗菌作用。VN 甲醇和己烷提取物显示存在重要的植物化学物质，如萜类、多酚、类固醇、皂苷、酚类化合物和类黄酮。VN 树皮甲醇提取物的总酚含量为 1082.473 mg/g GAE，叶为 1382.984 mg/g GAE。VN 树皮甲醇提取物的总类黄酮含量为 127.744 mg/g QE，叶为 123.776 mg/g QE。VN 甲醇提取物具有较高的抗氧化作用（IC50=38.47 ppm）。VN 叶中的灰分、水分、粗纤维素、粗蛋白和脂肪的含量分别为 7.86%、18.35%、6.52%、9.687%和 6.19%。VN 叶甲醇提取物对枯草芽孢杆菌和金黄色葡萄球菌具有抗菌活性，抑菌圈分别为 13mm 和 14mm，MBC 值分别为 1.562mg/mL 和 6.25mg/mL。同样，VN 树皮甲醇提取物对枯草芽孢杆菌和金黄色葡萄球菌的抑菌圈分别为 18mm 和 15mm，MBC 值分别为 2.372mg/mL 和 0.245mg/mL。VN 树皮甲醇提取物的 GC-MS 分析表明，单油酸甘油酯是主要化合物，面积为 0.57%。因此，我们的结果鼓励将 VN 作为一种具有高蛋白含量、显著抗氧化和抗菌作用的药用产品使用。
