Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, BE1410, Brunei.
F1000Res. 2020 Mar 13;9:187. doi: 10.12688/f1000research.21310.2. eCollection 2020.
is a popular ethnomedicinal plant but scientific studies to validate its pharmacological properties are lacking for this plant. This study aims to determine the antioxidant, antimicrobial and wound healing properties of the methanolic extract of the leaves and the hexane, chloroform and ethyl acetate fractions. The leaves of underwent methanol extraction and the methanol extract was fractionated with hexane, chloroform and ethyl acetate solvents. The antioxidant activity was determined using the DPPH radical scavenging assay. The antimicrobial activity was assessed by disc diffusion assay against and . For the wound healing studies, the methanolic extracts of were used to prepare ointments with compositions of 10% (w/w) and 50% (w/w), which were evaluated for wound healing activity in an excision wound model in Wistar rats. All the extracts showed antioxidant activities, with the ethyl acetate extract having the highest DPPH radical scavenging activity, followed by the methanol, chloroform and hexane extracts. Similarly, their quercetin equivalent concentrations were 33.1, 31, 20.3 and 4.5 mg/mL, respectively. Except for the methanol extract, the disc diffusion assay showed that the extracts demonstrated species-specific antibacterial activities, with the ethyl acetate extract showing antibacterial activities against all four tested strains. The wound healing activity of the high dose treated group (50% [w/w]) shows significant increase of wound contraction when compared to the control group. : In the current study, the ethyl acetate extract showed activity for all tested bacteria and also had the highest DPPH activity. The methanolic extracts of leaves show modest wound healing activity in an excision wound model.
是一种常用的民间药用植物，但缺乏对其药理特性的科学研究。本研究旨在确定该植物叶的甲醇提取物及其正己烷、氯仿和乙酸乙酯馏分的抗氧化、抗菌和伤口愈合特性。对 进行甲醇提取，用正己烷、氯仿和乙酸乙酯溶剂对甲醇提取物进行分级。采用 DPPH 自由基清除法测定抗氧化活性。采用圆盘扩散法评估对 和 的抗菌活性。对于伤口愈合研究，使用 的甲醇提取物制备组成分别为 10%（w/w）和 50%（w/w）的软膏，在 Wistar 大鼠切除伤口模型中评估其伤口愈合活性。所有提取物均表现出抗氧化活性，其中乙酸乙酯提取物的 DPPH 自由基清除活性最高，其次是甲醇、氯仿和正己烷提取物。同样，它们的槲皮素当量浓度分别为 33.1、31、20.3 和 4.5mg/mL。除甲醇提取物外，圆盘扩散试验表明，提取物表现出对所有测试菌株的特定抗菌活性，其中乙酸乙酯提取物对所有四种测试菌株均表现出抗菌活性。高剂量治疗组（50%[w/w]）的伤口愈合活性与对照组相比，伤口收缩显著增加。结论：在本研究中，乙酸乙酯提取物对所有测试的细菌均有活性，且 DPPH 活性最高。 的甲醇提取物在切除伤口模型中显示出适度的伤口愈合活性。
