Rozanov A Ia, Slizovskaia K
Ukr Biokhim Zh. 1977 Mar-Apr;49(2):45-8.
When incubating isolated erythrocytes, leucocytes and blood plasma 35S-lipoate in rising concentrations (2.4-240 microng/ml) the intensive binding of the latter may be observed, that is 8-11% of 35S-lipoate total content in the incubation medium for proteins, 4.8-08% for erythrocytes and 1.1-0.12% for leucocytes. Calculating per 10(7) cells the binding of lipoate by leucocytes is 1-2 order higher than that by erythrocytes. In blood plasma binding of 35S-lipoate by leucocytes is 41-82% lower than in the physiological solution. The binding of lipoate by erythrocytes also drops in the presence of plasma as compared to the physiological solution, but this effect is less pronounced and significant only with the presence of 240 microng of lipoate per 1 ml of the medium. Kinetics of 35-S-lipoate binding by the blood cells is of complicated character and permits supposing the existence of the lipoate-barrier function of the plasmatic membranes which is able of regulating lipoate intake by erythrocytes up to a concentration of 24 microng/ml and by leucocytes even up to 240 microng/ml.
当以递增浓度（2.4 - 240微克/毫升）孵育分离出的红细胞、白细胞和血浆中的35S - 硫辛酸时，可观察到后者的强烈结合，即孵育培养基中蛋白质结合的35S - 硫辛酸总量的8 - 11%，红细胞结合的为4.8 - 8%，白细胞结合的为1.1 - 0.12%。按每10⁷个细胞计算，白细胞对硫辛酸的结合比红细胞高1 - 2个数量级。在血浆中，白细胞对35S - 硫辛酸的结合比在生理溶液中低41 - 82%。与生理溶液相比，在血浆存在的情况下红细胞对硫辛酸的结合也会下降，但这种效应不太明显，只有在每1毫升培养基中存在240微克硫辛酸时才显著。血细胞对35 - S - 硫辛酸的结合动力学具有复杂的特点，这使得人们推测质膜存在硫辛酸屏障功能，它能够调节红细胞对硫辛酸的摄取，浓度可达24微克/毫升，对白细胞甚至可达240微克/毫升。
