Department of Pharmacology, SRM College of Pharmacy (Faculty of Medicine and Health Sciences), SRM Institute of Science and Technology (SRMIST) (Formerly SRM University), Kattankulathur, Chengalpattu District, Chennai - 603203, Tamilnadu, India.
Department of Pharmacology, SRM College of Pharmacy (Faculty of Medicine and Health Sciences), SRM Institute of Science and Technology (SRMIST) (Formerly SRM University), Kattankulathur, Chengalpattu District, Chennai - 603203, Tamilnadu, India.
J Ethnopharmacol. 2020 Oct 28;261:113070. doi: 10.1016/j.jep.2020.113070. Epub 2020 Jun 23.
Prosopis cineraria (L.) Druce is a plant that is widely found in dry parts of India. The unripe fruit pod has a very specific traditional claim of treating male infertility and increasing sperm volume and count.
The present work was endeavored to investigate the long-standing traditional claim of P. cineraria on meliorating male fertility. The study focussed on cancer therapy-induced male infertility and curative effect of the extract with an appraisal on any possible revitalizing effects on sperm count, morphology, motility, and viability combined with hormonal and histopathological investigations.
Male Wistar rats were used for the study. Two different doses of 400 mg/kg/d and 800 mg/kg/d (both p.o.) of the Hydroalcoholic extract were chosen as test dose while Clomiphene (25 mg/kg/d; p.o.) treatment served as standard treatment. Animals were initially injected with cisplatin (1 mg/kg/d; i.p.) for 15 days and the drug treatment was begun at the 16 day and continued till 43 day (28 days treatment). Later all male animals got cohabited with female animals in the ratio 1:3. On confirmation of mating, female animals were isolated. Male animals were euthanized on batches. Testis and epididymis were weighed and homogenized. Sperm count, motility, morphology, viability, and headcount. The serum collected was evaluated for serum FSH, LH, and testosterone levels. On day Gestational day 15, gravid uterus observations were calculated to evaluate male and female fertility parameters.
There were statistically significant improvements (p < 0.001) in sperm motility, sperm count, sperm viability, and improved morphological features. The same pace was also noticed in testosterone, FSH and LH levels in serum and LPO, CAT, GSH, GPx and SOD in testicular tissues. The extract treated male animals produced better and healthy litter compared to cisplatin-treated animals with less pre- and post-implantation loss.
Consolidating the results seen, the extract ameliorated the testicular toxicity caused by cisplatin in a dose-dependent manner. Further insight and evaluation of the phytochemicals of the pods should be performed to bring up commercial viability.
普那菊（Prosopis cineraria）（L.）Druce 是一种广泛分布于印度干燥地区的植物。未成熟的果实豆荚具有一种非常特殊的传统功效，即治疗男性不育症和增加精子数量和计数。
本研究旨在调查普那菊对改善男性生育力的长期传统功效。该研究集中于癌症治疗引起的男性不育症，并评估提取物的疗效，以及对精子计数、形态、活力和活力的任何可能的恢复作用，同时进行激素和组织病理学研究。
雄性 Wistar 大鼠用于本研究。选择两种不同剂量的 400 mg/kg/d 和 800 mg/kg/d（均为口服）的水醇提取物作为测试剂量，而氯米酚（25 mg/kg/d；口服）治疗作为标准治疗。动物最初接受顺铂（1 mg/kg/d；腹腔注射）治疗 15 天，在第 16 天开始药物治疗，并持续至第 43 天（28 天治疗）。之后，所有雄性动物与雌性动物以 1:3 的比例进行交配。在确认交配后，将雌性动物隔离。雄性动物分批安乐死。称重并匀浆睾丸和附睾。评估精子计数、活力、形态、活力和头计数。收集的血清用于评估血清 FSH、LH 和睾酮水平。在妊娠第 15 天，观察妊娠子宫以评估雄性和雌性生育力参数。
精子活力、精子计数、精子活力和形态特征均有统计学显著改善（p<0.001）。血清中睾酮、FSH 和 LH 水平以及睾丸组织中的 LPO、CAT、GSH、GPx 和 SOD 水平也有同样的改善。与顺铂处理的动物相比，用提取物处理的雄性动物产生的幼仔更好、更健康，着床前和着床后损失较少。
综合观察到的结果，提取物以剂量依赖的方式改善了顺铂引起的睾丸毒性。应该进一步深入研究和评估豆荚的植物化学物质，以提高商业可行性。
