Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, PR China.
Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, PR China.
Fish Shellfish Immunol. 2020 Sep;104:506-516. doi: 10.1016/j.fsi.2020.06.017. Epub 2020 Jun 22.
The TRAF family member-associated nuclear factor (NF)-κB activator (TANK) was first identified as a TRAF-binding protein with both stimulatory and inhibitory properties in host innate immune activation. To elucidate the roles of TANK in teleosts, we cloned and characterized the TANK homologue of orange-spotted grouper (Epinephelus coioides). The open reading frame (ORF) of EcTANK consists of 1026 nucleotides encoding a 342 amino acid protein with a predicted molecular mass of 38.24 kDa. EcTANK shares 89.47% and 88.89% identity with Larimichthys crocea TANK and Lates calcarifer TANK, respectively. EcTANK was distributed in all 11 examined tissues. The expression of EcTANK in the spleen increased after infection with Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV). EcTANK was mainly located in the cytoplasm of grouper spleen cells. EcTANK enhanced SGIV and RGNNV replication during viral infection in vitro. Overexpression EcTANK decreased the expression levels of interferon-associated cytokines and pro-inflammatory factors, and enhanced activation of NF-κB. Taken together, these results suggest that EcTANK may play an important role in antiviral innate immune activation in grouper.
TRAF 家族成员相关核因子（NF）-κB 激活物（TANK）最初被鉴定为一种 TRAF 结合蛋白，在宿主固有免疫激活中具有刺激和抑制双重特性。为了阐明 TANK 在硬骨鱼类中的作用，我们克隆并鉴定了尖吻鲈（Epinephelus coioides）的 TANK 同源物。EcTANK 的开放阅读框（ORF）由 1026 个核苷酸组成，编码一个 342 个氨基酸的蛋白质，预测分子量为 38.24 kDa。EcTANK 与 Larimichthys crocea TANK 和 Lates calcarifer TANK 的同源性分别为 89.47%和 88.89%。EcTANK 在 11 种检测组织中均有分布。EcTANK 在感染新加坡石斑鱼虹彩病毒（SGIV）和红鳍东方鲀神经坏死病毒（RGNNV）后在脾脏中的表达增加。EcTANK 主要位于石斑鱼脾细胞的细胞质中。EcTANK 在体外病毒感染过程中增强了 SGIV 和 RGNNV 的复制。过表达 EcTANK 降低了干扰素相关细胞因子和促炎因子的表达水平，并增强了 NF-κB 的激活。综上所述，这些结果表明 EcTANK 可能在鱼类抗病毒固有免疫激活中发挥重要作用。
